Sequences from confirmation amplicon


Confirmation sequences for line 404A01 and hit At4g12080 are shown.
Line ID 404A01
Locus At4g12080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7241234
Primers used for PCR 06vx - 8474, show primer details
   
Sequence Name 57-K118152-0022-404-A01C-06vx
GenBank Accession LN509540 [GenBank]
Sequencing primer gene specific primer, 06vx: 5'-AGTAGAACGGGAGGAATGAGTGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K118152-0022-404-A01C-06vx
GCCTCGCCGGTTTACTAGTAGCCGCGAGTCCGGTTCAGGTACGATATTTGCCAGTCCAAA
AAGCTGCAAAGTTTGTTAACCGGAGGATTTAATTTCCTTGTGTTTGAAATCAACAGGTGG
TTGTAGGAAGTTTTTTAGCGGGCACTGACCATCAAGATCAGAAACCGAAAAAGAACAAAC
ATGATTTCATGTTGTCGAGTCCTACCGCTGCAATTCCTATCTCTAGTGCAGCTGATCACC
GGACAATCCATTCGGTCTCGTCTCTTCCGGTCAATAATAATACATGGCAGACTTCTTTAG
CTTCCGATCCAAGAAACAAGCATACCGATATTAATGTCCAATACATGATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGC
GTTATT
   
Sequence Name 09-K118151-0022-404-A01C-8409
GenBank Accession LN490390 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K118151-0022-404-A01C-8409
TCGGATGAGCCATTTACATTGAATATATCATGTATTGGACATTAATATCGGTATGCTTGT
TTCTTGGATCGGAAGCTAAAGAAGTCTGCCATGTATTATTATTGACCGGAAGAGACGAGA
CCGAATGGATTGTCCGGTGATCAGCTGCACTAGAGATAGGAATTGCAGCGGTAGGACTCG
ACAACATGAAATCATGTTTGTTCTTTTTCGGTTTCTGATCTTGATGGTCAGTGCCCGCTA
AAAAACTTCCTACAACCACCTGTTGATTTCAAACACAAGGAAATTAAATCCTCCGGTTAA
CAAACTTTGCAGCTTTTTGGACTGGCAAATATCGTACCTGAACCGGACTCGCGGCTACTA
GTAAACCGGCGAGGCCACCGCCTACTACACGTCCATCGGGACTTGCTAACGATACACTCA
TTCCTCCCGTTCTACT

Last Updated on 06.12.2018 16:11