Sequences from confirmation amplicon


Confirmation sequences for line 404D06 and hit At4g39220 are shown.
Line ID 404D06
Locus At4g39220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18264346
Primers used for PCR TU03 - 8474, show primer details
   
Sequence Name 11-K026965-022-404-D06C-8409
GenBank Accession LN490960 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K026965-022-404-D06C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATATGCCTCATGTGCTCGCTGTTGA
ACAGGTGTGGCCACTGAACCACTATCACCTCCGCTTTCGTCCATCTAATGTGATATTTAA
AACCTAAAGGATCAACAAAACAGTTGGTTGGTCAAATTCACATATCTCTGCACCTTATCT
ACACAATTAACAACCCCAGGTTTTGTTCCTAACATACAAGATTACACATAATCTCTTCCC
ATATAATAGTAATTGGACACTAGAAGATTTTATCCAAGTATTTTCACTGGGAGATGAACA
CAGATGTATTTTCCCAGAAAATCATGTACGGAATCGCCTCTGAACACAACCGAAACTACT
TAAGAGTGGAGAAGAAAACTAACCTAGGAAACAAACAAATTTGTAGAAAACGCCATAAAA
TGTTTATCAGTCTAATGTATCTGACCAGAGAATCAAAACCTTAGTAACAACAGCAACTAT
CTCAATCGTAATCACCGATTGAACTTCAGATCTAGAAACTCAATCGGCGAAAACGATTCC
AACAAGCACATAACAAAAACCTTGA
   
Sequence Name 12-K026965-022-404-D06C-TU03
GenBank Accession LN491417 [GenBank]
Sequencing primer gene specific primer, TU03: 5'-TAGATCGACGATTCTCAAGGTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K026965-022-404-D06C-TU03
TGTTATGTGCTTGTTGGAATCGTTTTCGCCGATTGAGTTTCTAGATCTGAAGTTCAATCG
GTGATTACGATTGAGATAGTTGCTGTTGTTACTAAGGTTTTGATTCTCTGGTCAGATACA
TTAGACTGATAAACATTTTATGGCGTTTTCTACAAATTTGTTTGTTTCCTAGGTTAGTTT
TCTTCTCCACTCTTAAGTAGTTTCGGTTGTGTTCAGAGGCGATTCCGTACATGATTTTCT
GGGAAAATACATCTGTGTTCATCTCCCAGTGAAAATACTTGGATAAAATCTTCTAGTGTC
CAATTACTATTATATGGGAAGAGATTATGTGTAATCTTGTATGTTAGGAACAAAACCTGG
GGTTGTTAATTGTGTAGATAAGGTGCAGAGATATGTGAATTTGACCAACCAACTGTTTTG
TTGATCCTTTAGGTTTTAAATATCACATTAGATGGACGAAAGCGGAGGTGATAGTGGTTC
AGTGGCCACACCTGTTCAACAGCGAGCACATGAGGCATATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGA

Last Updated on 06.12.2018 16:11