Sequences from confirmation amplicon


Confirmation sequences for line 404F07 and hit At5g03840 are shown.
Line ID 404F07
Locus At5g03840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1024798
Primers used for PCR MM48 - 8474, show primer details
   
Sequence Name 21-K020131-022-404-F07C-MM48
GenBank Accession LN495474 [GenBank]
Sequencing primer gene specific primer, MM48: 5'-CCTATCAAGCCATGTATGAGGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K020131-022-404-F07C-MM48
CAACTTAGATTTTATTAATTAATCAAGAGTTTACTATTTTTGTAGTATTACAATGCTAAA
CATGATATGCAATTAATCTAGTTTTTATTCTGATAATCGACAGATCGTTATCAATACTTC
TGTAAACTCAATTGATATTTCGGATTCAACTCATCTTTGGCAGTTTTTATTCAGAGTACG
GAAATTCAGAACACAAATAAACATGAGATTACATGCAAATTTTCTTCTTTCATTTTTGCA
GTTTATGACAATCATGAAACTAGCGTTTGCGTGCAGCGGTTTCTCTTTGTGCGTTAAAAT
ATGTCCGGGAAACCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 22-K020131-022-404-F07C-8409
GenBank Accession LN495921 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K020131-022-404-F07C-8409
TACATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGTTTCCCGGACATATT
TTAACGCACAAAGAGAAACCGCTGCACGCAAACGCTAGTTTCATGATTGTCATAAACTGC
AAAAATGAAAGAAGAAAATTTGCATGTAATCTCATGTTTATTTGTGTTCTGAATTTCCGT
ACTCTGAATAAAAACTGCCAAAGATGAGTTGAATCCGAAATATCAATTGAGTTTACAGAA
GTATTGATAACGATCTGTCGATTATCAGAATAAAAACTAGATTAATTGCATATCATGTTT
AGCATTGTAATACTACAAAAATAGTAAACTCTTGATTAATTAATAAAATCTAAGTTGCTG
TAGTATATAAATCATTAAATCCTCATACATGGCTTGATAG

Last Updated on 06.12.2018 16:11