Sequences from confirmation amplicon


Confirmation sequences for line 406E07 and hit At1g22980 are shown.
Line ID 406E07
Locus At1g22980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8132806
Primers used for PCR 11sr - 8474, show primer details
   
Sequence Name 63-K116549-0022-406-E07C-11sr
GenBank Accession LN511067 [GenBank]
Sequencing primer gene specific primer, 11sr: 5'-GTTTCGGTTATCATGAAGGATGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K116549-0022-406-E07C-11sr
AGGGTGAGGCATCCGGTTGAGATTTTTTCTCCCTGAGCAAATAGAAGTTTGCTAAAATGG
TTGCTGACATTGTGTATGAGGCGATGACGGTAATAATAGTCATTCGAGTAATCACCAGGA
TGATGGAGAAGGAGAATTCAAATGAGAATAGCGTGTTTGTGGAGTCGCTAGAGAAGCTAC
TTAAGTTGTGTCAAAGAAGTGGAGTAGTGATAGAAGAGCTTGGTACTTGCGTTTATCATC
CACCGCTGAAGATAGACAAGATAACTCAAACGGTGAAGATACTAGAGGGAAATCTCGATG
AAGTTGAGGCTCAAGTTGAGTATATGAAGAGGTCTTCCAACGCCTTTCCGGGAGTTTGCA
GAAAGCTGCGAGACGCGATCAAACTCATGGAAGTTGGATTGGAAAAAAGAAAGGATTTGA
ATCAAATTCTGATTTCTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGAT
   
Sequence Name 23-K116548-0022-406-E07C-8409
GenBank Accession LN496710 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K116548-0022-406-E07C-8409
GAAATCAGAATTCGATTCAAATCCCTTTCTTTTTTTCAATCCAACTTCCATGAGTTTGAT
CGCGTCTCGCAGCTTTCTGCAAACTCCCGGAAAGGCGTTGGAAGACCTCTTCATATACTC
AACTTGAGCCTCAACTTCATCGAGATTTCCCTCTAGTATCTTCACCGTTTGAGTTATCTT
GTCTATCTTCAGCGGTGGATGATAAACGCAAGTACCAAGCTCTTCTATCACTACTCCACT
TCTTTGACACAACTTAAGTAGCTTCTCTAGCGACTCCACAAACACGCTATTCTCATTTGA
ATTCTCCTTCTCCATCATCCTGGTGATTACTCGAATGACTATTATTACCGTCATCGCCTC
ATACACAATGTCAGCAACCATTTTAGCAACTTCTATTTGCTCAGGAGAAAAATCATCACC
AGATGCCTCACCCTCACTTGAAGGACAAGCTGGTTTCACTTTTTTCATCTCATTAAGAAC
ATCCTT

Last Updated on 06.12.2018 16:11