Sequences from confirmation amplicon


Confirmation sequences for line 408A01 and hit At4g28890 are shown.
Line ID 408A01
Locus At4g28890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14256801
Primers used for PCR 38uw - 8474, show primer details
   
Sequence Name 77-K117671-0022-408-A01C-38uw
GenBank Accession LN513602 [GenBank]
Sequencing primer gene specific primer, 38uw: 5'-GGAGCTTTACATCGAGAGAGAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K117671-0022-408-A01C-38uw
CTTAGTGGGTCGTCGAGATTCGCATCGGAGAGAGTTTCCGGAAGATTCTGAAATTAGGTA
ATAAAGAAAAAACGTTACTTGATGAACACGTGAACGATAAAGATGAGAAGAAGCTTATGC
ACAAGTTCAATCATAGGATCGTTGTGTCGGATGTTGTTTTCAAGAATCGTTGGAGCAACG
TGAGTTCATCAGATTTGATGTTTTTGAATTCCGAGATGGTTAATTCGATTTCTAGCGAGA
GATTTTCTTCATTGGATCATGTGAAGAGAGGAGATGAAGAGGATCAGATAGGAAATCCTA
TCAGGATTTTCTTCTGGGATATCAGTGTTTAAACTATCAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 29-K117670-0022-408-A01C-8409
GenBank Accession LN499461 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K117670-0022-408-A01C-8409
ATTTCATTGAATAATCCTGATAGTTAAACACTGATATCCCAGAAGAAAATCCTGATAGGA
TTTCCTATCTGATCCTCTTCATCTCCTCTCTTCACATGATCCAATGAAGAAAATCTCTCG
CTAGAAATCGAATTAACCATCTCGGAATTCAAAAACATCAAATCTGATGAACTCACGTTG
CTCCAACGATTCTTGAAAACAACATCCGACACAACGATCCTATGATTGAACTTGTGCATA
AGCTTCTTCTCATCTTTATCGTTCACGTGTTCATCAAGTAACGTTTTTTCTTTATTACCT
AATTTCAGAATCTTCCGGAAACTCTCTCCGATGCTGAATCTCGACGACCCACTAAGTTCT
TCTCTGTGTATTCTTTCTTCTTCTTCTTCTCTCTCGATGTAAAGCTCC

Last Updated on 06.12.2018 16:11