Sequences from confirmation amplicon


Confirmation sequences for line 408E11 and hit At3g18980 are shown.
Line ID 408E11
Locus At3g18980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6546709
Primers used for PCR 08ij - 8474, show primer details
   
Sequence Name 35-K112667-0022-408-E11C-08ij
GenBank Accession LN501889 [GenBank]
Sequencing primer gene specific primer, 08ij: 5'-ATATTTGGCCGGTCCTACTTATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K112667-0022-408-E11C-08ij
CGTAACTGATAGATCTTCCTTGGACAAATCTACTTCCGATAATGTTGATTCCCTTCTGTC
TGAATTCTCTCTAGTGTAAAAAATATTCTCTTGAATCCTTGGTTTTCCATAGCGTGTGTG
TATTGATTTACAAATATAGAATCTACAGAAAACAAGATTGAGGGTGCAAAAATGAAAATA
TATTCAGAATTGAGGGAGCAATTTGTTACATTTCCTCTTTCCAGTCTATGTATATATCTC
GCAAGAGGAAAGCTGAAGAAAAGCAAAACCCTAGAAAATAAATAATATTCCGTGGCGCCT
GCGGCAGAGATCAATGACGATACCGGATCTCTGTAACGATTTGGTCGATGAGATACTCTG
TCGCGTTCCGGCGAGGAATCTGAAACGGTTACGATCTACCAGCAAACGATGGAACCGTTT
ATTCAAAGATGATAGGAGATTCGCAAGAGAGCACATGCATAAAGCCCCAAAGGAGTATCT
ACCTCTCATGTTGACAAGCGAGTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 03-K112666-0022-408-E11C-8409
GenBank Accession LN487556 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K112666-0022-408-E11C-8409
TCATTGAATATTCCTGTACTCGCTTGTCAACATGAGAGGTAGATACTCCTTTGGGGCTTT
ATGCATGTGCTCTCTTGCGAATCTCCTATCATCTTTGAATAAACGGTTCCATCGTTTGCT
GGTAGATCGTAACCGTTTCAGATTCCTCGCCGGAACGCGACAGAGTATCTCATCGACCAA
ATCGTTACAGAGATCCGGTATCGTCATTGATCTCTGCCGCAGGCGCCACGGAATATTATT
TATTTTCTAGGGTTTTGCTTTTCTTCAGCTTTCCTCTTGCGAGATATATACATAGACTGG
AAAGAGGAAATGTAACAAATTGCTCCCTCAATTCTGAATATATTTTCATTTTTGCACCCT
CAATCTTGTTTTCTGTAGATTCTATATTTGTAAATCAATACACACACGCTATGGAAAACC
AAGGATTCAAGAGAATATTTTTTACACTAGAGAGAATTCAGACAGAAGGGAATCAACATT
ATCGGAAGTAGATTTTGTCCAAGGAAGATCTATCAGTTTACGCTGACGTGTATGACATTA
TTACTGTACTTTAGATAAGTAGGACCGGCCAAATA

Last Updated on 06.12.2018 16:11