Sequences from confirmation amplicon


Confirmation sequences for line 409B01 and hit At2g37260 are shown.
Line ID 409B01
Locus At2g37260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15646708
Primers used for PCR 07pl - 8474, show primer details
   
Sequence Name 36-K115038-0022-409-B01C-07pl
GenBank Accession LN502337 [GenBank]
Sequencing primer gene specific primer, 07pl: 5'-TCTAGAGGATTTCGATAGACCACC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K115038-0022-409-B01C-07pl
AATTGCATTATGTTTAGCAACGTGAACAGAAAAGAAATGGTCCCATGAAGTCAAATTTCT
CAAGTTCGATAAACTAAACATATTGAAGAAAGTTACCCCTAAAAAATATAACACACGTTT
TTATTTGGATTTGTTAAAATAACATCAATCTTCTTGTCCTTACAATCTTAATGTAAAAAC
AAATAAATTCACACGATTAATTAACTAATGTGGTATAAAGATTGAAGAAACAGGCATGAA
TTGATCAAATGGCTTTTGCTTAGGAAGTTGTGAGTGAAGAGCAAATGGTGATTGTATTTA
CCATTTGCTTCTTCTTTTTTCTCATTCTTAACAAAAAAAGAAAAAGAAAAAAGAAAAAGC
ATGAATGGATCATCAAATCAAATTGTTTGCTTAGAAAGTTGTGGGGAGTTAAAGGGAAGA
GTAGAGGAAGATGGAGGGCTCAAGAGCAAATGATGATTATGTCTTTCTTTTTAGTATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCC
   
Sequence Name 26-K115037-0022-409-B01C-8409
GenBank Accession LN498053 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K115037-0022-409-B01C-8409
TTGAATACTAAAAAGAAAGACATAATCATCATTTGCTCTTGAGCCCTCCATCTTCCTCTA
CTCTTCCCTTTAACTCCCCACAACTTTCTAAGCAAACAATTTGATTTGATGATCCATTCA
TGCTTTTTCTTTTTTCTTTTTCTTTTTTTGTTAAGAATGAGAAAAAAGAAGAAGCAAATG
GTAAATACAATCACCATTTGCTCTTCACTCACAACTTCCTAAGCAAAAGCCATTTGATCA
ATTCATGCCTGTTTCTTCAATCTTTATACCACATTAGTTAATTAATCGTGTGAATTTATT
TGTTTTTACATTAAGATTGTAAGGACAAGAAGATTGATGTTATTTTAACAAATCCAAATA
AAAACGTGTGTTATATTTTTTAGGGGTAACTTTCTTCAATATGTTTAGTTTATCGAACTT
GAGAAATTTGACTTCATGGGACCATTTCTTTTCTGTTCACGTTGCTAAACATAATGCAAT
TTCCATCGGTGTAACTCAATTACATTGGTGGTCTAT

Last Updated on 06.12.2018 16:11