Sequences from confirmation amplicon


Confirmation sequences for line 409D02 and hit At5g42810 are shown.
Line ID 409D02
Locus At5g42810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17168001
Primers used for PCR 16ij - 8474, show primer details
   
Sequence Name 53-K112831-0022-409-D02C-16ij
GenBank Accession LN508204 [GenBank]
Sequencing primer gene specific primer, 16ij: 5'-TTGATTAGGTTGGGAAAGTGATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K112831-0022-409-D02C-16ij
GCATCAGAATGGAATGGTGTTGTTTCGGTTTTAACAGCGATGAGCAACATCTATGGAGAG
AAAACAATGAGCTGATTTCATCACCAAACAAGGAAGTTCTTGAACAAAGATATGTTAAGA
ATGTGATCATCCCACTCTTGGGTCCTAAACATGTTGATGCTGGAGTAAGTAATATTATCA
AATGGTCTTTGTTGGTTTTATTTAGATACTTATGGCTTCTTGGTAAGCTGATGATTAGAG
TATTGTAGGTACGTGTTTCTGTGTCCAAGGAGTTTCTTGAGTGTGTTGATAAGAAAGTAA
CTAAGCAGCGTCCGCTATGGCGTGTTAATGCAGCAAATGTTGATACGAGTCATGATTCTG
CCCTTATATTGAATGATCATTCACTTTTTTCTCAAGGTAACCTATTATTATAAGAAATCG
GACCACATCTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGTTATTATAATNNTAGGTTACCTTGAGAAAAAAGTGAAT
GATCATTCAATATAAGGGCAGAATCATGACTCGTATCAACATTTGCTGCATTAACACGCC
ATAGCGGACGCTGCTTAGTTACTTTCTTATCAACACACTCAAGAAACTCCTTGGACACAG
AAACACGTACCTACAATACTCTAATCATCAGCTTACCAAGAAGCCATAAGTATCTAAATA
AAACCAACAAAGACCATTTGATAATATTACTTACTCCAGCATCAACATGTTTAGGACCCA
AGAGTGGGATGATCACATTCTTAACATATCTTTGTTNCAGAACTTCCTTGTTTGGTGATG
AAATCAGCTCATTG
   
Sequence Name 24-K112829-0022-409-D02C-8409
GenBank Accession LN497065 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K112829-0022-409-D02C-8409
CATTTCATTGAATATGATGTGGTCCGATTTCTTATAATAATAGGTTACCTTGAGAAAAAA
GTGAATGATCATTCAATATAAGGGCAGAATCATGACTCGTATCAACATTTGCTGCATTAA
CACGCCATAGCGGACGCTGCTTAGTTACTTTCTTATCAACACACTCAAGAAACTCCTTGG
ACACAGAAACACGTACCTACAATACTCTAATCATCAGCTTACCAAGAAGCCATAAGTATC
TAAATAAAACCAACAAAGACCATTTGATAATATTACTTACTCCAGCATCAACATGTTTAG
GACCCAAGAGTGGGATGATCACATTCTTAACATATCTTTGTTCAAGAACTTCCTTGTTTG
GTGATGAAATCAGCTCATTGTTTTCTCTCCATAGATGTTGCTCATCGCTTGTTAAAACCG
AAACAACACCATTCGCATTCTTGATTGCTTTATCATTCCTCCGAGCCTTCTGTATACGAA
TCACTTTCCCAANCTAATCA

Last Updated on 06.12.2018 16:11