Sequences from confirmation amplicon


Confirmation sequences for line 409E07 and hit At1g64610 are shown.
Line ID 409E07
Locus At1g64610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24000530
Primers used for PCR CB69 - 8474, show primer details
   
Sequence Name 58-K112479-0022-409-E07C-CB69
GenBank Accession LN509748 [GenBank]
Sequencing primer gene specific primer, CB69: 5'-AAGGAAGAACAAAAGTACAGGCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K112479-0022-409-E07C-CB69
GAGTGAAGTTCTAGACATTCATCACAACCTACTCTCCATAAACGAACAGTCTCGTCAACA
GAAGATGACAAGAGATACTGCACCAAAGAGAGGTAAATGTAAGAGGATGGGCACAATCAG
AGACTATATAAATGTAGGGAAGAGTTTGAAAAGATGTTTACCCCTTTCTCCGACCAGGAA
AGATCCAAGATCTCACCTATATGTCCTTTGAACTCATGCAGAGGCTTCTCCGATATACTG
AAGATGGTAGGAGGTAATACAACACAAGTTGAGTCTGATGACTTCCTTAAAAAACTTGAC
TTCTTTTCAGTTTTCTCATTGTTGATCTTCAGGGGCTCAATCTGAGAACGTTGATTCATC
CCAAAGTAAACACCAGAATCAACCTCAGCTACTTCAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTA
   
Sequence Name 28-K112478-0022-409-E07C-8409
GenBank Accession LN498913 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K112478-0022-409-E07C-8409
CATTTACATTGAATTATCCTGAAGTAGCTGAGGTTGATTCTGGTGTTTACTTTGGGATGA
ATCAACGTTCTCAGATTGAGCCCCTGAAGATCAACAATGAGAAAACTGAAAAGAAGTCAA
GTTTTTTAAGGAAGTCATCAGACTCAACTTGTGTTGTATTACCTCCTACCATCTTCAGTA
TATCGGAGAAGCCTCTGCATGAGTTCAAAGGACATATAGGTGAGATCTTGGATCTTTCCT
GGTCGGAGAAAGGGGTAAACATCTTTTCAAACTCTTCCCTACATTTATATAGTCTCTGAT
TGTGCCCATCCTCTTACATTTACCTCTCTTTGGTGCAGTATCTCTTGTCATCTTCTGTTG
ACGAGACTGTTCGTTTATGGAGAGTAGGTTGTGATGAATGTCTTAGAACTTTCACTCATA
ACAATTTCGGTGAGCTTCTTCTTCAGTATATTGCCTGTACTTTTGTTCTTCCT

Last Updated on 06.12.2018 16:11