Sequences from confirmation amplicon


Confirmation sequences for line 409F05 and hit At4g11230 are shown.
Line ID 409F05
Locus At4g11230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6841212
Primers used for PCR ME45 - 8474, show primer details
   
Sequence Name 33-K027640-022-409-F05C-8409
GenBank Accession LN500859 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K027640-022-409-F05C-8409
ATTGAATATGTCCGGGAAACGACAATCTGAGTATAGTGAGAAGATTGGATCGAGTATCTC
CTTCTTCGTAGACGCTAGTCAAGTAGTTGTGCATCTCAATAACACCCTTGTAGTTGTTCA
CAAGACAAATACGTCAGTGAGTAACTTGAAATCATACAATCTTTTGAATTTAAAATGAAT
AAGTGTATTGTTTTTACCTTTCTATCTGAATCAGCAATCTCGTTCATGATTTCTTTGAAC
CAATCGAACGAGCCTTGTTCTCGTGTTACCCAGTAAAAGTAAGCATTCTTGGTATTCAGC
GTCTTCCGTTGAGTTTGCGGAATCCGACTCGCAGCTTCTGAGTTTAAGCAACTGAAACTG
TGATCCGACGAGATGTTGCTGTTACTACAACTACCTGAGATGCATTCCTGAGAATATAAC
ATAACTCCAAAAAAGTTTAGTTGAGATTCTGTTACTCATCAACAAGGTTTTTAAGCTTGT
TATACTCACAGCTTGTTCCTGTTGCTTAATAATGTTATTGAGTAGATCTCTC
Primers used for PCR ME45 - 8474, show primer details
   
Sequence Name 35-K112877-0022-409-F05C-8409
GenBank Accession LN501896 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K112877-0022-409-F05C-8409
TTACATTGAATATGTCCGGGAAACGACAATCTGAGTATAGTGAGAAGATTGGATCGAGTA
TCTCCTTCTTCGTAGACGCTAGTCAAGTAGTTGTGCATCTCAATAACACCCTTGTAGTTG
TTCACAAGACAAATACGTCAGTGAGTAACTTGAAATCATACAATCTTTTGAATTTAAAAT
GAATAAGTGTATTGTTTTTACCTTTCTATCTGAATCAGCAATCTCGTTCATGATTTCTTT
GAACCAATCGAACGAGCCTTGTTCTCGTGTTACCCAGTAAAAGTAAGCATTCTTGGTATT
CAGCGTCTTCCGTTGAGTTTGCGGAATCCGACTCGCAGCTTCTGAGTTTAAGCAACTGAA
ACTGTGATCCGACGAGATGTTGCTGTTACTACAACTACCTGAGATGCATTCCTGAGAATA
TAACATAACTCCAAAAAAGTTTAGTTGAGATTCTGTTACTCATCAACAAGGTTTTTAAGC
TTGTTATACTCACAGCTTGTTCCTGTTGCTTAATAATGTTATTGAGTAGATCTCTCAAAA
TGCTNACGAAAGG
   
Sequence Name 72-K112878-0022-409-F05C-ME45
GenBank Accession LN512749 [GenBank]
Sequencing primer gene specific primer, ME45: 5'-ACCTTTCGTTAGCATTTTGAGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K112878-0022-409-F05C-ME45
TAGCACGGAACAGCTGTGAGTATAACAAGCTTAAAACCTTGTTGATGAGTAACAGAATCT
CAACTAAACTTTTTTGGAGTTATGTTATATTCTCAGGAATGCATCTCAGGTAGTTGTAGT
AACAGCAACATCTCGTCGGATCACAGTTTCAGTTGCTTAAACTCAGAAGCTGCGAGTCGG
ATTCCGCAAACTCAACGGAAGACGCTGAATACCAAGAATGCTTACTTTTACTGGGTAACA
CGAGAACAAGGCTCGTTCGATTGGTTCAAAGAAATCATGAACGAGATTGCTGATTCAGAT
AGAAAGGTAAAAACAATACACTTATTCATTTTAAATTCAAAAGATTGTATGATTTCAAGT
TACTCACTGACGTATTTGTCTTGTGAACAACTACAAGGGTGTTATTGAGATGCACAACTA
CTTGACTAGCGTCTACGAAGAAGGAGATACTCGATCCAATCTTCTCACTATACTCAGATT
GTCGTTTCCCGGACATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCACGGTTA

Last Updated on 06.12.2018 16:11