Sequences from confirmation amplicon


Confirmation sequences for line 411B06 and hit At4g39250 are shown.
Line ID 411B06
Locus At4g39250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18271649
Primers used for PCR IM19 - 8474, show primer details
   
Sequence Name 31-K032519-022-411-B06C-8409
GenBank Accession LN500049 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K032519-022-411-B06C-8409
TTACTGACCGTTCTCTATGCTGTTGATGTCTTGCACTAAGAGTTCGTAGTGTCTCTTTAC
CTCCTCTGTTGTTTTTCCACCCACGACTTTGGCAACATTTTGCCAACGGTTAGGAGTGTC
CTGGTCATAGGTTGCTAGAGCCTGCTCAAAGGCTTTGTTCTGCTTAGCAGTCCACGAGCC
AGAGCTCTGAGAAGACATTGAGCTTGATGCCATGTTGAGTTATAGACTAATATAACTGGT
TAGAAAGAGATTATTTAAGTATGGGGAGATTACGAGTCAAGAGGATGATATGTGCATANG
ATGTTGCAG
   
Sequence Name 31-K032519-022-411-B06C-IM19
GenBank Accession LN500050 [GenBank]
Sequencing primer gene specific primer, IM19: 5'-TCTGCAACATCTATGCACATATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K032519-022-411-B06C-IM19
TAATATCTCTTTCAACCAGTTATATTAGTCTATAACTCAACATGGCATCAAGCTCAATGT
CTTCTCAGAGCTCTGGCTCGTGGACTGCTAAGCAGAACAAAGCCTTTGAGCAGGCTCTAG
CAACCTATGACCAGGACACTCCTAACCGTTGGCAAAATGTTGCCAAAGTCGTGGGTGGAA
AAACAACAGAGGAGGTAAAGAGACACTACGAACTCTTAGTGCAAGACATCAACAGCATAG
AGAACGGTCATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTACATTCACT

Last Updated on 06.12.2018 16:11