Sequences from confirmation amplicon


Confirmation sequences for line 411E01 and hit At3g16480 are shown.
Line ID 411E01
Locus At3g16480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5599931
Primers used for PCR 14tg - 8474, show primer details
   
Sequence Name 79-K116920-0022-411-E01C-14tg
GenBank Accession LN513872 [GenBank]
Sequencing primer gene specific primer, 14tg: 5'-TTCGTTTCACTTTACACACACCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K116920-0022-411-E01C-14tg
AAAAGAAAAGAAAAAAAATATAATCGAGAGAAAATATAATAGGATCACACCACTACAATA
CATCGGGCTTTAAATTCATCTTTTCTATATGTTATAATTTGGGCCAAGACTATTATATCA
TATGGGCCTATCAATGTAATTAGAAGCAATTGCCCGATCCAATAACCTTAATTAGGAAAA
AAAATCGTTCCCTTCTCTCGTTTCTTTTCAGCTCTGACTCGGCCTCTATCTCTCTCCCGT
CGTCAGCCACATTCACCGGTACTCATCTGACCAAGCTAGGGTTTCGCAGTCATCATCATG
TACCGTACGGCAGCTTCTCGAGCCAAGGCCCTTAAGGTCAATGTCCTACTTCACTTGTTT
CCATTGAGATCTACATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
   
Sequence Name 35-K116919-0022-411-E01C-8409
GenBank Accession LN501974 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K116919-0022-411-E01C-8409
GCTCGAGAAGCCGCCGTACGGTACACGACGACGACCGCCAAACCCTAGCTCGGTCAGAAG
AGTACCGGTGAACGCGGCCGACGACCGGAGAGAGATAGAGGCCGAGTCAGAGCTGAAAAG
AAACGAGAGAAGGGAACGATTTTTTTTCCTAATTAAGGTTATTGGATCGGGCAATTGCTT
CTAATTACATTGATAGGCCCATATGATATAATAGTCTTGGCCCAAATTATAACATATAGA
AAAGATGAATTTAAAGCCCATGTATTGTAGTGGTGTGATCCTATTATATTTTCTCTCAAT
TATATTTTTTTTCTTTTCTTTTTCACGTACCATTGTGTAAAAATTCAAGTAAGGTAACGA
CATTTACATGCAGTCACAAATGAAACAAGAATTTTTTTGAGGTGTGTGTA

Last Updated on 06.12.2018 16:11