Sequences from confirmation amplicon


Confirmation sequences for line 411H07 and hit At2g34370 are shown.
Line ID 411H07
Locus At2g34370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14510614
Primers used for PCR BJ52 - 8474, show primer details
   
Sequence Name 27-K115418-0022-411-H07C-BJ52
GenBank Accession LN498528 [GenBank]
Sequencing primer gene specific primer, BJ52: 5'-CAACAAAGATTCATCAGTGTTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K115418-0022-411-H07C-BJ52
CATTCTCTCACAAAATCCAACGCCTCATCCAATGACCACAAGCTGCCAACATCTCAATTA
CATTCACATAGTCTTCCATTGAAAGAACCATACCATAATCTCTGTACATAGACTCAAAAT
GCAACAATCCCTCGTTAATGTCTCCTATCGAAACACACGCAAAGAAAACAGCTTTGAATA
TCTCCTTGTCGGGTTTATTCCCTTCCTCTATGAAACGCGTGAACATATCAATTGCGCGTT
CACCTTCTCCATTCTTCGCTAAGCACCTTATCATTGTACCCCAAGTCTCTGAGTTTCTCT
TAGGCATTTCGTTGAACACATTCAAAGCGTCATCAGTGGATCGACAACCAGAATACATTT
CGATTACCGTGTGGTAAGATCTAGCATCTAAAGGAGTGATGCAGTCATGAACAACCCTAG
CTTCTTCCAAAGCCTCTACTTCACCACATAGTTTCGCTAACCCTAAAAGCCTGGGAAAAT
CCACTATATATCCCTTATCCTCCAAGATATCAATAACTTCCAAGGCTTCTCTTATCTTCA
CTTGTTTACACAAAGCATCAAATGTCTCAATCGTCACAGATTGATGATTATTAACCATCT
GAGCATAGGAAGAGACTCTTCTGCATTGAACAAAGCTTCGATTTTGCCAAATGGGACGTG
TTAAAACGTCTTGAAGAGACTTCGTCTACATGACTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGT
   
Sequence Name 20-K115417-0022-411-H07C-8409
GenBank Accession LN495345 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K115417-0022-411-H07C-8409
TCTCTTCAGACGTTTTACACGTCCCATTTGGCAAAATCGAAGCTTTGTTCAATGCAGAAG
AGTCTCTTCCTATGCTCAGATGGTTAATAATCATCAATCTGTGACGATTGAGACATTTGA
TGCTTTGTGTAAACAAGTGAAGATAAGAGAAGCCTTGGAAGTTATTGATATCTTGGAGGA
TAAGGGATATATAGTGGATTTTCCCAGGCTTTTAGGGTTAGCGAAACTATGTGGTGAAGT
AGAGGCTTTGGAAGAAGCTAGGGTTGTTCATGACTGCATCACTCCTTTAGATGCTAGATC
TTACCACACGGTAATCGAAATGTATTCTGGTTGTCGATCCACTGATGACGCTNTGAATGT
GTTCAACGAAATGCCTAAGAGAAACTCAGAGACTTGGGGTACAATGATAAGGTGCTTAGC
GAAGAATGGAGAAGGTGAACGCGCAATTGATATGTTCACGCGTTTCATAGAGGAAGGGAA
TAAACCCGACAAGGAGATATTCAAAGCTGTTTTCTTTGCGTGTGTTTCGATAGGAGACAT
TAACGAGGGATTGTTGCATTTTGAGTCTATGTACAGAGATTATGGTATGGTTCTTTCAAT
GGAAGACTATGTGAATGTAATTGAGATGTTGGCAGCTTGTGGTCATTTGGATGAGGCGTT
GGATTTTGTTGAGAGAATGACTGTGGAACCGAGTGTTGAAATGTGGGAAACACTGATGA

Last Updated on 06.12.2018 16:11