Sequences from confirmation amplicon


Confirmation sequences for line 412C05 and hit At3g57854 are shown.
Line ID 412C05
Locus At3g57854 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21425516
Primers used for PCR 24rb - 8474, show primer details
   
Sequence Name 53-K117914-0022-412-C05C-24rb
GenBank Accession LN508287 [GenBank]
Sequencing primer gene specific primer, 24rb: 5'-GTGATCAAAGCCTACCTGAGTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K117914-0022-412-C05C-24rb
ACGTTACGGCCAGATCTTACCGNTTACTATTGCATAATAAGAACATGAGTTCAGATAGTA
ATTTTTTTTGTATATAACTATTATCAGTTTATCACCATATATAATATGTTTATTAGTCCC
AAGCATAACAAAGATCATATTCTCGAGTCTGTGCATCAAGAGCACAAGCTCCATACACTA
TGATGGACCAAAATCAGTTTCAGCACTGATATTTGTCAATATTATCTCTGCTATCTTTAT
AAATAGTAATACGTTGTGCTTTATGACCAAATATAAACATACAAACGTTACGAGTTAGTC
CCCGTAAACTTTTCCTAAACTGAACGAATTCCGTCGTACGCCCCGGTAAAAATACTTGGT
CTTAATAATTCCCATTACTACGACAACTAGAATTTAGCACCAAACCATCTCCGATATCGT
TTGTGATGTATACATATATTTTCAAAAGAAAAATCCGATTTGAATATATTCTCACCCATC
ACTACACCGTTTTGAGATAATGCTACTTTCATAAGCAAGGATAGTATCACAAGCATTATC
ACAACCATTTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACAATTATA
   
Sequence Name 13-K117913-0022-412-C05C-8409
GenBank Accession LN492237 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K117913-0022-412-C05C-8409
TATCCTTGCTTAGAAGTAGCATTATCTCAAACGGTGTAGTGATGGGTGAGAATATATTCA
AATCGGATTTTTCTTTTGAAAATATATGTATACATCACAAACGATATCGGAGATGGTTTG
GTGCTAAATTCTAGTTGTCGTAGTAATGGGAATTATTAAGACCAAGTATTTTTACCGGGG
CGTACGACGGAATTCGTTCAGTTTAGGAAAAGTTTACGGGGACTAACTCGTAACGTTTGT
ATGTTTATATTTGGTCATAAAGCACAACGTATTACTATTTATAACGATAGCAGAGATCAT
ATTGACAAATATTACTGCTGAAACTGATTTTGGTCCATCATAGTGTATGGAGCTTGTGCT
CTTGATGCACAGACTCGAGAATATGATCTTTGTTATGCTTGGGACTAAAAAACATATTAT
ATATGGTGATAAACTGATAATAGTTATATACAAAAAAAATTACTATCTGAACTCATGTTC
TTATGATGCAATATGTAACGGGAAGATCTGTGCCGTTGAACGGTTGGATCGGTGAGGCGT
AAAGAATCTCAGGTA

Last Updated on 06.12.2018 16:11