Sequences from confirmation amplicon


Confirmation sequences for line 412G11 and hit At5g07440 are shown.
Line ID 412G11
Locus At5g07440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2356737
Primers used for PCR OB02 - 8474, show primer details
   
Sequence Name 29-K024136-022-412-G11C-OB02
GenBank Accession LN499124 [GenBank]
Sequencing primer gene specific primer, OB02: 5'-CGAGAGAAGTCTTATGATCCCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K024136-022-412-G11C-OB02
AAATCAAGGTTCGACTTTTTTTTTTCCTTTTGAGGTTTTACTCTTTTGTTTCGTGTTTTG
GGCGAAAATTCGTCTCAATCTTTGTTTGGATTTTTTTACTTTTGATTCCAGGTTGAGTGA
ACAATTCCTAAAAACGATGGCACTCTGGTTTCATACATCGGATTTAGGGTTCAACATGAC
AATGCTCGTGGACCCATGAAAGGTGGAATCAAATATCATCCTGAGGTAGGGATCTTTTAC
CATTTGTTCTTGATCTAGGATTTAAAGTTGACAACTTTGGGGGTTATAGATTGAATCTAG
GGATTTGAATTAAGGGTTTTGATTTTTTGGTTTGAAGGTCGACCCAAATGAATTTAACCC
ACTAC
   
Sequence Name 30-K024136-022-412-G11C-8409
GenBank Accession LN499560 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K024136-022-412-G11C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATTCAATTTAAACACACG
AGTCAACCTCTCAAGCTCGCTCAAACTCAAGTCACGAGGACTACATCCAATTCCACCTTT
AGCACCACCATATGGAATATCTGCTACAGCAGTCTTCCAAGTCATCAGCTGAGCTAGTGC
GTTAACTTCATCTGGATCAACCTTCAAACCAAAAAATCAAAACCCTTAATTCAAATCCCT
AGATTCAATCTATAACCCCCAAAGTTGTCAACTTTAAATCCTAGATCAAGAACAAATGAT
AAAAGACCCTACCTCAGGATGATATCTGATTCCACCTTTCATGGGTCCACGAGCATTGTC
ATGTTGAACCCTAAATCCGATGTATGAAACCAGAGTGCCATCGTCTTTAGGAATCGTACA
CTCAACCTGAAATCAAAAGTAAAAAAATCCAAACAAAGATTGAGACGAATTTTCCCCAAA
AACGAAACAAAAGAGTAAAACCTCAAAAGGAAAAAAAAAAGTCAAACCTTGATTTCTCTA
AATGGGATCA

Last Updated on 06.12.2018 16:11