Sequences from confirmation amplicon


Confirmation sequences for line 413B02 and hit At4g38360 are shown.
Line ID 413B02
Locus At4g38360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17967745
Primers used for PCR 12gz - 8474, show primer details
   
Sequence Name 53-K112309-0022-413-B02C-12gz
GenBank Accession LN508198 [GenBank]
Sequencing primer gene specific primer, 12gz: 5'-AGAACTACAAGATGCGGAACCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K112309-0022-413-B02C-12gz
TGGTCATACCATGCAGGCAACAGGTATCTACCAAAACAGTACATGGCAAAGGATTCATAG
CAGTCTCGAAGAATCCCACAGTCAACACTGATGGATGGTTTAACAAGTGATGCAAACTGT
TACAAACAAACACAATTAGGAACAGATATCTTAGGGAAACACATTCAGTTGACGATCCGT
TGACGAGCACAATACAAGTAAATATTCGATGAGATTATCAACGCTGAACATGACTGTATT
ATTATGAGTATGATACAGGCTTCTTCTACACTTTGGCGATAAAAAAGTCAATTTTTTTAG
GTTCCAATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 37-K112308-0022-413-B02C-8409
GenBank Accession LN502665 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K112308-0022-413-B02C-8409
GCATTTCATTGATATATCCTGATTGGAACCTAAAAAATTGACTTTTTTATCGCCAAAGTG
TAGAAGAAGCCTGTATCATACTCATAATAATACAGTCATGTTCAGCGTTGATAATCTCAT
CGAATATTTACTTGTATTGTGCTCGTCAACGGATCGTCAACTGAATGTGTTTCCCTAAGA
TATCTGTTCCTAATTGTGTTTGTTTGTAACAGTTTGCATCACTTGTTAAACCATCCATCA
GTGTTGACTGTGGGATTCTTCGAGACTGCTATGAATCCTTTGCCATGTACTGTTTTGGTA
GATACCTTGTTGCCTGCATTGGTATGACCATTTTCACTCTTTTTTATGTCTAATATTGAT
TAGGTTCCGCATCTTGTAGTTC

Last Updated on 06.12.2018 16:11