Sequences from confirmation amplicon


Confirmation sequences for line 414B04 and hit At1g14500 are shown.
Line ID 414B04
Locus At1g14500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4961574
Primers used for PCR 50mk - 8474, show primer details
   
Sequence Name 51-K114277-0022-414-B04C-50mk
GenBank Accession LN507578 [GenBank]
Sequencing primer gene specific primer, 50mk: 5'-CTTCTTCTCGGTATCTCACTTGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K114277-0022-414-B04C-50mk
AGAACATGACATTCAATAACAATTTGTCGAGGTTATATCTTTAGATTCTCAAAGTCCCTC
TCTGTTTCACAAATGTAACAAACATTCGATTTACTGCAGCCTTCAGATGGCTCAGCCTCA
GGGCTATGAAAATTTCCATAAAGAGATACATAGGAAAGAGCAGGTACAAGGTAAAGGCCG
CACAAAGAAACAATAGAGGGTTGGGTGAGATTATAGCCATAGCAAGCGCATAAGAAACTC
CCAGAGAATAGATCAGCAATGTTGGAAATCTACATAGGTTTACTTCATGGTAGCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 21-K114276-0022-414-B04C-8409
GenBank Accession LN495759 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K114276-0022-414-B04C-8409
CTACCATGAAGTAAACCTATGTAGATTTCCACATTGCTGATCTATTCTCTGGGAGTTTCT
TATGCGCTTGCTATGGCTATAATCTCACCCAACCCTCTATTGTTTCTTTGTGCGGCCTTT
ACCTTGTACCTGCTCTTTCCTATGTATCTCTTTATGGAAATTTTCATAGCCCTGAGGCTG
AGCCATCTGAAGGCTGCAGTAAATCGAATGTTTGTTACATTTGTGAAACAGAGAGGGACT
TTGAGAATCTAAAGATATAACCTCGACAAATTGTTATTGAATGTCATGTTCTATGAGGTA
ATCAGCTTAATGAGTTTCATCGGGAGCCAAAGTTTAAAACTTTTAAAGCAAGTGAGATAC
GNAAGAAGAA

Last Updated on 06.12.2018 16:11