Sequences from confirmation amplicon


Confirmation sequences for line 414C11 and hit At4g15735 are shown.
Line ID 414C11
Locus At4g15735 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8963436
Primers used for PCR 31qv - 8474, show primer details
   
Sequence Name 74-K115779-0022-414-C11C-31qv
GenBank Accession LN513131 [GenBank]
Sequencing primer gene specific primer, 31qv: 5'-GTGGTGTTAAATTGCTGGGTCTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K115779-0022-414-C11C-31qv
CACCAAATTGATGTTATTGTTTTTTGTGAGTTTATTGATTTTTGAGGTAACATAGGCCGG
ATCTATTATTAGATAGGTAAAAAGGAATGGTCCCGATATATAGTTTCAGAAAAAACTGTG
AATAATTTTTGAGGTAAATCGGAGTTGGTACCGCCGTACCGGAATGATCTCGGAAAGCTA
AAAAAATATGACCACTTAAAATAATTCAATAGATGAATATGAGATCTAGGGTATTATTGG
GGACAAAATTTTTATTATAATTGTGCTGAAAATTAAGTAGATAACAACATATTAGTAGGT
GTTAATTTTTTACACAACAATCGGTGCAAATAACTGAACATTCACAAAGACGCTTGTTAT
TGGGCTGAGGGGTACAATCGCTCAAAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCACACGGTTATT
   
Sequence Name 26-K115778-0022-414-C11C-8409
GenBank Accession LN498068 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K115778-0022-414-C11C-8409
CATTTTTGAGCGATTGTACCCCTCAGCCCAATAACAAGCGTCTTTGTGAATGTTCAGTTA
TTTGCACCGATTGTTGTGTAAAAAATTAACACCTACTAATATGTTGTTATCTACTTAATT
TTCAGCACAATTATAATAAAAATTTTGTCCCCAATAATACCCTAGATCTCATATTCATCT
ATTGAATTATTTTAAGTGGTCATATTTTTTTAGCTTTCCGAGATCATTCCGGTACGGCGG
TACCAACTCCGATTTACCTCAAAAATTATTCACAGTTTTTTCTGAAACTATATATCGGGA
CCATTCCTTTTTACCTATCTAATAATAGATCCGGCCTATTTTACCTCAAAAATCAATAAA
CTCACAAAAAACAATAACATCAATTTTTGGTTGTGTAGAGGAGTTTTGACTATCTAATAA
TAGACCCAGCAATTTAACACCA

Last Updated on 06.12.2018 16:11