Sequences from confirmation amplicon


Confirmation sequences for line 414D10 and hit At3g62220 are shown.
Line ID 414D10
Locus At3g62220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23030017
Primers used for PCR 38jd - 8474, show primer details
   
Sequence Name 79-K113193-0022-414-D10C-38jd
GenBank Accession LN513849 [GenBank]
Sequencing primer gene specific primer, 38jd: 5'-TACCCATGTGACTAGACTTTGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K113193-0022-414-D10C-38jd
CTTTCACCTGTAGAAGCTCGAGCAGTACAACTCCGAAACTGTATACGTCACTCTTGGCAC
TCAATTGCCCAGTCATTGCATATCTAGTAAAGAGAATACGAGCATATGAGAGATCGAGGA
TTGAACAATGGAGAATAAGAAGAAAAGAAAACCAACTTACTCAGGGGCATGGTAACCAAA
GGTTCCAAGAACACGAGTTGAATGAAGGCGTGCTGCCATATCAGGAGCTTGATTTGAGAG
ATCAAAGTCAGCTATCTTGGCTACATCGTTATCAAAGATTAGAACATTGCTGGATTTTAT
CAAAGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCACGNTTATT
   
Sequence Name 42-K113192-0022-414-D10C-8409
GenBank Accession LN504526 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K113192-0022-414-D10C-8409
CATTTCATTGAATATATCCTGACTTTGATAAAATCCAGCAATGTTCTAATCTTTGATAAC
GATGTAGCCAAGATAGCTGACTTTGATCTCTCAAATCAAGCTCCTGATATGGCAGCACGC
CTTCATTCAACTCGTGTTCTTGGAACCTTTGGTTACCATGCCCCTGAGTAAGTTGGTTTT
CTTTTCTTCTTATTCTCCATTGTTCAATCCTCGATCTCTCATATGCTCGTATTCTCTTTA
CTAGATATGCAATGACTGGGCAATTGAGTGCCAAGAGTGACGTATACAGTTTCGGAGTTG
TACTGCTCGAGCTTCTTACAGGTCGAAAGCCTGTTGATCATACATTGCCTCGAGGCCAGC
AAAGTCTAGTCACATGGGTA

Last Updated on 06.12.2018 16:11