Sequences from confirmation amplicon


Confirmation sequences for line 414G04 and hit At1g01010 are shown.
Line ID 414G04
Locus At1g01010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3702
Primers used for PCR 51mk - 8474, show primer details
   
Sequence Name 32-K114225-0022-414-G04C-51mk
GenBank Accession LN500675 [GenBank]
Sequencing primer gene specific primer, 51mk: 5'-TACCACATAGCATCTCTCGATTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K114225-0022-414-G04C-51mk
CTAAATCAAAACCTAAATCGATTACTTGCAAATGAAATTCAGAAAATTCGGAACTTACAG
CGCAAGTTCCAAGGATCGTAGCTACAGATGTTGACCTCGCTGATGGCTACTTCAACGTCG
CGGCTAGTGTTTCCTTCGATTTTGTTACGGAGATAGTGACCAACGAGCTCCTCGTCGTTC
GGACGGAACCCAAACCCAACTTGATCCTCCATTTTCACTAAACCGTCCGATTTCAATGGT
TTCCGGGTAAAGAGCTTCGCCGTCGATCTCAATTGTAAATTCGATTATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACAGGTTA
   
Sequence Name 12-K114224-0022-414-G04C-8409
GenBank Accession LN491672 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K114224-0022-414-G04C-8409
ATTTCATTGAAAATCGAATTTACAATTGAGATCGACGGCGAAGCTCTTTACCCGGAAACC
ATTGAAATCGGACGGTTTAGTGAAAATGGAGGATCAAGTTGGGTTTGGGTTCCGTCCGAA
CGACGAGGAGCTCGTTGGTCACTATCTCCGTAACAAAATCGAAGGAAACACTAGCCGCGA
CGTTGAAGTAGCCATCAGCGAGGTCAACATCTGTAGCTACGATCCTTGGAACTTGCGCTG
TAAGTTCCGAATTTTCTGAATTTCATTTGCAAGTAATCGATTTAGGTTTTTGATTTTAGG
GTTTTTTTTTGTTTTGAACAGTCCAGTCAAAGTACAAATCGAGAGATCTTATGTGGT

Last Updated on 06.12.2018 16:11