Sequences from confirmation amplicon


Confirmation sequences for line 415B04 and hit At3g49220 are shown.
Line ID 415B04
Locus At3g49220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18251245
Primers used for PCR 16nt - 8474, show primer details
   
Sequence Name 67-K114716-0022-415-B04C-16nt
GenBank Accession LN511872 [GenBank]
Sequencing primer gene specific primer, 16nt: 5'-TTTTTAAAACCCTATCACTCCGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K114716-0022-415-B04C-16nt
GGCCCCTTCAGTATCTGATTCCATAATTCTCCTCAAAGTATATATATTTTTCCTTCTTAA
CTTATTTATATCAATACNCTTTTTCAGTAACAAATATGTACAACAAATAGTTTATATTAA
ATCTATAGAAAAACGACATTTATTATATCATAAATGTTGACCCTCATAGCTCCTTTTTAA
GATTTTAATTAACACAAAAAGATCTTTTAACTTTTCTATTATATCACTAAAAAACGCTTC
TGATTTTCGACCACCGAAAAGATTAGTGTATCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 19-K114715-0022-415-B04C-8409
GenBank Accession LN494872 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K114715-0022-415-B04C-8409
TTGATACACTAATCTTTTCGGTGGTCGAAAATCAGAAGCGTTTTTTAGTGATATAATAGA
AAAGTTAAAAGATCTTTTTGTGTTAATTAAAATCTTAAAAAGGAGCTATGAGGGTCAACA
TTTATGATATAATAAATGTCGTTTTTCTATAGATTTAATATAAACTATTTGTTGTACATA
TTTGTTACTGAAAAAGGTATTGATATAAATAAGTTAAGAAGGAAAAATATATATACTTTG
AGGAGAATTATTGGAATCAGATACGTGAAGGGGGCCATGAGCTAGGCTGTCTATAGATTT
CGGAGTGATAGGGTTTTAAAA

Last Updated on 06.12.2018 16:11