Sequences from confirmation amplicon


Confirmation sequences for line 415E03 and hit At1g30270 are shown.
Line ID 415E03
Locus At1g30270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10656997
Primers used for PCR 36xe - 8474, show primer details
   
Sequence Name 37-K119409-0022-415-E03C-36xe
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 36xe: 5'-AGAACACTAGCCTTACCCCTGAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K119409-0022-415-E03C-36xe
ACTCTCAGGCTGACTCAGCATTCTCAAATTTAGGTGCTTTATACCCTTTTTTAACCACTC
TTTTTCAATGACCTCAGCAAATGTAATCCTCTGCCCAATATAAAAAATCAGTGTAAGCCT
TCAGATCTACTCAATCCTATAGAAGCTTTTGCCTCTTCAAAGGAGAGATCCTCTAATATA
AATTATATAATAGGGTAAGAAAAATATACCGTTGCTGGATTGGGATCCAGAATTCTTTTG
ATTAACTTCTTAGCACTTGCAGAGAACCAGGGAGGGCAAGTAAATTCTGCTTTGATAATG
GCTTGAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATTAT
   
Sequence Name 13-K119408-0022-415-E03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K119408-0022-415-E03C-8409
ATTTCAATTGAATATTAAGCCATTATCAAAGCAGAATTTACTTGCCCTCCCTGGTTCTCT
GCAAGTGCTAAGAAGTTAATCAAAAGAATTCTGGATCCCAATCCAGCAACGGTATATTTT
TCTTACCCTATTATATAATTTATATTAGAGGATCTCTCCTTTGAAGAGGCAAAAGCTTCT
ATAGGATTGAGTAGATCTGAAGGCTTACACTGATTTTTTATATTGGGCAGAGGATTACAT
TTGCTGAGGTCATTGAAAATGAGTGGTTTAAAAAAGGGTATAAAGCACCTAAATTTGAGA
ATGCTGATGTCAGCCTTGATGATGTTGATGCAATCTTTGATGACTCAGGGGTAAGGCTAG
TGTTCT

Last Updated on 06.12.2018 16:11