Sequences from confirmation amplicon


Confirmation sequences for line 415G01 and hit At2g21380 are shown.
Line ID 415G01
Locus At2g21380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9148264
Primers used for PCR 18dr - 8474, show primer details
   
Sequence Name 77-K110174-0022-415-G01C-18dr
GenBank Accession LN513564 [GenBank]
Sequencing primer gene specific primer, 18dr: 5'-AGCTGAAGAAGCAAAGAGAAGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K110174-0022-415-G01C-18dr
ACATGTGGGTGCTTGTTGCCAGTTGAAGAAAGCCATAGTGGCGCCTTGTCAATACAAAAG
AGTGACGAAGCAGAGCCTGCAAAGGAAGATGAAGTGACAGAGTTAGATAATAAGAATGAA
CAAAATGCAATCTTGAAAGAGAGGCAACTCGTGAATGGACATGAAGAAGTAATAGTTGCC
AAGGCTGAAGAGACACCAAAGGAGGAACCTCTTGTTGCTCGTCTTAAGGTAAACCTTTTA
CAAATTTGCTTTCTATTTTCTTTCAGCATTCTTAGTTTTCTATAAGCCTACTCCACAGTC
TAGCGGTAGGCAAATCTAGATATCCATTACCTGTTCGCTCCATATCCTTTATGCCATGAC
CTTCGGATCAGGCAACATATATTAAAACCTTCAAAACGAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
ACGATTATT
   
Sequence Name 67-K110173-0022-415-G01C-8409
GenBank Accession LN511836 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K110173-0022-415-G01C-8409
GTTGCCTGATCGAAGGTATGGCATAAAGGATATGGAGCGAACAGGTAATGGATATCTAGA
TTTGCCTACCGCTAGACTGTGGAGTAGGCTTATAGAAACCTAATAATGCCTGAAAGAAAA
TATAAAGCGAATTTGTAAAAGGTTTA

Last Updated on 06.12.2018 16:11