Sequences from confirmation amplicon


Confirmation sequences for line 415H01 and hit At3g01200 are shown.
Line ID 415H01
Locus At3g01200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:70558
Primers used for PCR 38sj - 8474, show primer details
   
Sequence Name 63-K116453-0022-415-H01C-38sj
GenBank Accession LN511066 [GenBank]
Sequencing primer gene specific primer, 38sj: 5'-TTTGAGTAGCAAATGAGAATGGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K116453-0022-415-H01C-38sj
ATCCGGGGTTCGCTCATAGATCCTTGACGCGAAATCTAGTTCTTTCAGAACAGATCAATC
CCCGAGTAATTGTTCTCTGCCTCTGTATCTACGCCTAGCGTTTTAACCCTTGTTCTTCTA
ATCGCTTGCAGTACAACGAGCTGGATCTTCAAACCAAAAACTTTTCTCGGTTCAACCTGA
AAGAGCGTCCTGGGTGGTTCCACGCCCATCACAAACGGCACATTAGCAACTTTGTACCCT
TTTTGAGCAATGTAAGTGGACAAGGGCGTCTTCCCTGTCCGGGAAACCCCGACAAGAACT
ATATCAGCCTTGCTTAAGTTCTCAGGTAAAGTCCCATCATCTTGCTTAATGGTAAACTCA
ATGGCTTCAATCCTTTTGAAATATGCATCATTGAGAGTCTTGACCCTACCAGGTGCTCCA
CGTGTAAGACCTGACGGAGAAACACCTAAGTGAGACGCAATCCCTTCGATGATGGGAAAT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCA
   
Sequence Name 30-K116452-0022-415-H01C-8409
GenBank Accession LN499872 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K116452-0022-415-H01C-8409
TTGATTTCCATCATCGAAGGGATTGCGTCTCACTTAGGTGTTTCTCCGTCAGGTCTTACA
CGTGGAGCACCTGGTAGGGTCAAGACTCTCAATGATGCATATTTCAAAAGGATTGAAGCC
ATTGAGTTTACCATTAAGCAAGATGATGGGACTTTACCTGAGAACTTAAGCAAGGCTGAT
ATAGTTCTTGTCGGGGTTTCCCGGACAGGGAAGACGCCCTTGTCCACTTACATTGCTCAA
AAAGGGTACAAAGTTGCTAATGTGCCGTTTGTGATGGGCGTGGAACCACCCAGGACGCTC
TTTCAGGTTGAACCGAGAAAAGTTTTTGGTTTGAAGATCCAGCTCGTTGTACTGCAAGCG
ATTAGAAGAACAAGGGTTAAAACGCTAGGCGTAGATACAGAGGCAGAGAACAATTACTCG
GGGATTGATCTTGTTCGGAAAGAACTAGATTTCGCGTCAAGGATCTATGAGCGAAACCCC
GGATGGGCAGTGATAGGTAAACAAAACTTAAACACCATTCTC

Last Updated on 06.12.2018 16:11