Sequences from confirmation amplicon


Confirmation sequences for line 416D01 and hit At4g19930 are shown.
Line ID 416D01
Locus At4g19930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10804684
Primers used for PCR JG70 - 8474, show primer details
   
Sequence Name 57-K034070-022-416-D01C-8409
GenBank Accession LN509338 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K034070-022-416-D01C-8409
GAAGCCATTTACAATTGAATATATCTTTAACCNGGAGAGGTTATCGTGGAACCTCAAAAT
ACAACTCTTTTATACATTTTTCTCTATGATTTACAAAAGAATCTGATGAGAAAAGTTGAA
ATTAAAGAACCACCATATCATACTAAATTTTGGGATGTTGTTGGGTTAGATGATGTTGAG
AACTTCATGTATCTTTAAATTCATCTAGCTTGAGCGGTTTTTTGTTTTTATGTTAAAAAT
TATTTTATTAACGAAGCTGACGACTGATACATAACACAAGCACAATCATTTTTGTTGTTT
AAGCATTTTAAGTTTCAACTGCTTTCTCTGTTGTGAGTTATGAAGCCAGTATTTGAAGCA
ATATGATTCAAAGAGTGCAACCAAAGATTGTTAACCCTCTCTCATCACCGTTTCAAGAAA
ACGTTTACCGAATAATCTTTCAAACATTTTCATATCTTCGGTCCAAACCAAAGGAGATTA
CAACGAGTTTTCCTTTCACTAGCTCAATCATTGATACTCACCTTCTCGGTGCAAACACCA
CTCTGACTCTGCGTCTCTCTGTTTTCAGTAGATACACACTATTTCCTAGGGCGTGGGAAC
AGTAAACCAAACCAAAAGAAAATAAACGGAACCAA
   
Sequence Name 73-K034070-022-416-D01C-JG70
GenBank Accession LN512842 [GenBank]
Sequencing primer gene specific primer, JG70: 5'-TTTGGTTCGTTTATTTTCTTTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K034070-022-416-D01C-JG61
TAGTGTGTATCTACTGAAAACAGAGAGACGCAGAGTCAGAGTGGTGTTTGCACCGAGAAG
GTGAGTATCAATGATTGAGCTAGTGAAAGGAAAACTCGTTGTAATCTCCTTTGGTTTGGA
CCGAAGATATGAAAATGTTTGAAAGATTATTCGGTAAACGTTTTCTTGAAACGGTGATGA
GAGAGGGTTAACAATCTTTGGTTGCACTCTTTGAATCATATTGCTTCAAATACTGGCTTC
ATAACTCACAACAGAGAAAGCAGTTGAAACTTAAAATGCTTAAACAACAAAAATGATTGT
GCTTGTGTTATGTATCAGTCGTCAGCTTCGTTAATAAAATAATTTTTAACATAAAAACAA
AAAACCGCTCAAGCTAGATGAATTTAAAGATACATGAAGTTCTCAACATCATCTAACCCA
ACAACATCCCAAAATTTAGTATGATATGGTGGTTCTTTAATTTCAACTTTTCTCATCAGA
TTCTTTTGTAAATCATAGAGAAAAATGTATAAAAGAGTTGTATTTTGAGGTTCCACGATA
ACCTCTCCGGTTAAAGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
AACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATT
CGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11