Sequences from confirmation amplicon


Confirmation sequences for line 418A04 and hit At1g61105 are shown.
Line ID 418A04
Locus At1g61105 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22514264
Primers used for PCR NU06 - 8474, show primer details
   
Sequence Name 51-K113934-0022-418-A04C-NU06
GenBank Accession LN507571 [GenBank]
Sequencing primer gene specific primer, NU06: 5'-AAACAAAGTGGCAAAATATCCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K113934-0022-418-A04C-NU06
AAGACACAAACCATAGCTAACTATAAGCGTAAAACGATCACTTATAAATATAGAGACACA
TATCTATTTCTTACTAATCTAAAATCATTTCTTCTTCCACAATACTAAAAGCAATTATCA
AAAATATATCAAAACCAGTCTTCATCTCTAAATCACTCTTTCGCTTAATTCAATCATTTC
ATTTCTTCCTCGTCTATTCAAAACTCTTCGATGCAACGTTTGTTAACACCTTGCTCAAAA
ATCGCATACACAACAAGATACATTGCAAATCCAACCCTACCTTACCCAAACCCATGTGAT
ATTTTCATCAATCATCGTGGGATCGATACCAAGAAGACTATTTCAGGCCTACTATATGAT
TGTTTCACTAGACAACGTTTGACTGCTTTCTTGGATAGTAAGAGTTTAAAGCCTGGAGAC
AGGCTTTTTGTCGAGATCGACATGGCAATCAAAGCATGCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGC
   
Sequence Name 16-K113933-0022-418-A04C-8409
GenBank Accession LN493511 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K113933-0022-418-A04C-8409
CGATCTCGACAAAAAGCCTGTCTCCAGGCTTTAAACTCTTACTATCCAAGAAAGCAGTCA
AACGTTGTCTAGTGAAACAATCATATAGTAGGCCTGAAATAGTCTTCTTGGTATCGATCC
CACGATGATTGATGAAAATATCACATGGGTTTGGGTAAGGTAGGGTTGGATTTGCAATGT
ATCTTGTTGTGTATGCGATTTTTGAGCAAGGTGTTAACAAACGTTGCATCGAAGAGTTTT
GAATAGACGAGGAAGAAATGAAATGATTGAATTAAGCGAAAGAGTGATTTAGAGATGAAG
ACTGGTTTTGATATATTTTTGATAATTGCTTTTAGTATTGTGGAAGAAGAAATGATTTTA
GATTAGTAAGAAATAGATATGTGTCTCTATATTTATAAGTGATCGTTTTACGCTTATAGT
TAGCTATGGTTTGTGTCTTTGTGAATAAGGTCCGAGAACTCCATGTCGGATCTTGGATAT
TTTGCCACTTTGTT

Last Updated on 06.12.2018 16:11