Sequences from confirmation amplicon


Confirmation sequences for line 418E05 and hit At4g02650 are shown.
Line ID 418E05
Locus At4g02650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1158629
Primers used for PCR 40by - 8474, show primer details
   
Sequence Name 76-K108065-0022-418-E05C-40by
GenBank Accession LN513412 [GenBank]
Sequencing primer gene specific primer, 40by: 5'-GTCTACGGCTTATGGAAGTAGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K108065-0022-418-E05C-40by
AGCCAGCAGGTAAACAAATTTAAAATGAAATCATTTTTCAATTTTTATCTTAGTTTCATG
AGAGTCAGTTCAATGAATCTGATCTAAATGATGATGCAGCCTCGATGTTGGCATTGCCAG
CGCCGCCACCGACAGCCAATGGAAACAGAAATAGTCCGGTGATGGTGGATCCATTCGCGG
CGTCGCTAGAGGTAGCCCCACCAGCATACGTGCAAATGAATGATATGGAGAAAAAACAAA
GATTGTTAATGGAAGAACAAATAATGTGGGATCAATACAACAGAAGTGGAAGACAAGGTC
ACATGAATTTCGGACAAAACCAACAACAACAATATTATCAGCTTCCATACTCCATGGGAC
CTTACTCTTACACGCCTCGCTATTGATATTATATAAATAAACAATCACCATTAATGAAGA
GCTTGAAAGAAAGGTGTTAGCTTCTTTTTTGTTGTCCCTCTAACATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 06-K108066-0022-418-E05C-8409
GenBank Accession LN488845 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K108066-0022-418-E05C-8409
TTTACATGTTAGAGGGACAACAAAAAGAAGCTAACACCTTTCTTTCAGCTCTTCATTAAT
GGTGATTGTTTATTTATATAATATCAATAGCGAGGCGTGTAAGAGTAAGGTCCCATGGAG
TATGGAAGCTGATAATATTGTTGTTGTTGGTTTTGTCCGAAATTCATGTGACCTTGTCTT
CCACTTCTGTTGTATTGATCCCACATTATTTGTTCTTCCATTAACAATCTTTGTTTTTTC
TCCATATCATTCATTTGCACGTATGCTGGTGGGGCTACCTCTAGCGACGCCGCGAATGGA
TCCACCATCACCGGACTATTTCTGTTTCCATTGGCTGTCGGTGGCGGCGCTGGCAATGCC
AACATCGAGGCTGCATCATCATTTAGATCAGATTCATTGAACTGACTCTCATGAAACTAA
GATAAAAATTGAAAAATGATTTCATTTTAAATTTGTTTTACCTGCTGGGCTTCCTGCAGA
ACCAAAAGCCACACTACTAGCACTTCCACTACTTCCATAAGCCGTAGA

Last Updated on 06.12.2018 16:11