Sequences from confirmation amplicon


Confirmation sequences for line 418F12 and hit At5g67310 are shown.
Line ID 418F12
Locus At5g67310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26855799
Primers used for PCR AY31 - 8474, show primer details
   
Sequence Name 40-K020068-022-418-F12C-8409
GenBank Accession LN503556 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K020068-022-418-F12C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAAGCCATTTACTCGCTTCTTTC
TTTTAAACAGAAGGGCGTGTGTTTGAGGAATCAGATACTGGCAAGTGTCCCTATCTCAAC
AATGTGATCTCTGAGACTCTTCGTTTGTTTCCGGCGGCGCCCTTACTTGTCCCACACGCT
TCATCCACTGACTGTGAAGTCGCTGGATTTGACATTCCACGTCGCACCTGGCTATTTATC
AATGCGTGGGCTATTCAGAGAGACCCAAATGTATGGGATGACCCAGAAACTTTCAAACCA
GAGAGGTTTGAATCAGAGACCCATCGTGGTAAGTTTCTGCCTTTCGGAATCGGTAGGAGA
GCATGTCCTGGTATGGGCCTGGCCCAACTAGTACTGAGCTTAGCTTTAGGCTCATTGATC
CAGTGTTTCGATTGGGAGAGAGACAATGACGTTGCCGTTGACATGTCGGAAGGAAAGGGT
CTTACCATGCCCAAATCTGGCCTTTATTGCCAAATGCAAGTCTTTACCAATCC
   
Sequence Name 39-K020068-022-418-F12C-AY31
GenBank Accession LN503199 [GenBank]
Sequencing primer gene specific primer, AY31: 5'-AAGTTTGTCGAGGATTGGTAAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K020068-022-418-F12C-AY31
CTTGCATTTGGCAACTAAAGGCACAGATTTGGGCATGGTAAGACCCTTTCCTTCCGACAT
GTCAACGGCAACGTCATTGTCTCTCTCCCAATCGAAACACTGGATCAATGAGCCTAAAGC
TAAGCTCAGTACTAGTTGGGCCAGGCCCATACCAGGACATGCTCTCCTACCGATTCCGAA
AGGCAGAAACTTACCACGATGGGTCTCTGATTCAAACCTCTCTGGTTTGAAAGTTTCTGG
GTCATCCCATACATTTGGGTCTCTCTGAATAGCCCACGCATTGATAAATAGCCAGGTGCG
ACGTGGAATGTCAAATCCAGCGACTTCACAGTCAGTGGATGAAGCGTGTGGGACAAGTAA
GGGCGCCGCCGGAAACAAACGAAGAGTCTCAGAGATCACATTGTTGAGATAGGGACACTT
GCCAGTATCTGATTCCTCAAACACACGCCCTTCTGTTTAAAAGAAAGAAGCGAGTAAATG
GCTTCAATTGTAAATGGCTTCATGTCCGGAAATCTACATGGATCGCAATGAGTATGATGG
TCAATATNGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAGTACATTTTGATAAAACGACAAATTACGATCCGTC
GTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGT
GTCTAC

Last Updated on 06.12.2018 16:11