Sequences from confirmation amplicon


Confirmation sequences for line 418H02 and hit At1g60490 are shown.
Line ID 418H02
Locus At1g60490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22289126
Primers used for PCR MJ36 - 8474, show primer details
   
Sequence Name 27-K029911-022-418-H02C-8409
GenBank Accession LN498253 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K029911-022-418-H02C-8409
ATTGAATATATCCTGCCGCCGATGTTCAAAACTACAGAAATCAATGACCACAAACAGATG
TGAGCTTCCATGTTTGGTGCTTTCTTGCTCCTTAATCGTATCCAAGGATTTCAACATAAG
TCGATCCAGCCAATCTATGCTTTGAATCTGCCCCCTCTCATACTTATTCATGAGCTTCTC
TAAACGCTCTAGCTCTCCACGCTCATGCCGTGGAACCTACGAGTACAGAAAAATAACAGA
TAACCCAAGAAAAAGTAGGTCGGCATCAAGAACGATATTTCGGATTATATAGGAAGAGTT
GAGATGTATCAGAAACCTTTCCAGGAGTAGAGGTTGGAAATGAACCATCCGCTTCTTTCC
CCTGCCAGAGCCTTAGCTTCTGCTTCCCTGATTTCATTTGCATCTTGGCTGT
   
Sequence Name 27-K029911-022-418-H02C-MJ36
GenBank Accession LN498254 [GenBank]
Sequencing primer gene specific primer, MJ36: 5'-TAACAGCAAGATGCAAATGAAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K029911-022-418-H02C-MJ36
CGGAGGTTCATTTCCACCTCTACTCCTGGAAAGGTTTCTGATACATCTCAACTCTTCCTA
TATAATCCGAAATATCGTTCTTGATGCCGACCTACTTTTTCTTGGGTTATCTGTTATTTT
TCTGTACTCGTAGGTTCCACGGCATGAGCGTGGAGAGCTAGAGCGTTTAGAGAAGCTCAT
GAATAAGTATGAGAGGGGGCAGATTCAAAGCATAGATTGGCTGGATCGACTTATGTTGAA
ATCCTTGGATACGATTAAGGAGCAAGAAAGCACCAAACATGGAAGCTCACATCTGTTTGT
GGTCATTGATTTCTGTAGTTTTGAACATCGGCGGCAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11