Sequences from confirmation amplicon


Confirmation sequences for line 419G07 and hit At1g21880 are shown.
Line ID 419G07
Locus At1g21880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7681772
Primers used for PCR NS40 - 8474, show primer details
   
Sequence Name 92-K102437-0022-419-G07C-8409
GenBank Accession LN515186 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K102437-0022-419-G07C-8409
TTACAATTGAATATATCCTGCAGCTATTATTTGGGAGCCGCAAGTAATTATATCTAAGCT
TTATTATTTGATATGACTAATCAAATTCATCTCTGATTGCATTTTGACTCACTTGTGTTG
TGTGTGGGTGATGTGATGCAGTTTGTATTGTGAGCCTGCTTCCCTAGCCGTCTCTTGCTC
AAGTATGCAGTGTAGAAACAGCAACCTCATGCTCGGTAACATCACGGTGCAGCAGACTAG
CGCTGGCTGTAATGTTACAACTTGTGATTACAACGGTATAGCCAACGGCACTATCTTAAC
CATGTAAGTAGAAATACATTCACTTTGTCTTCTCCTCTTATCAACATACCCCTACTAATG
ATTCGAAACTATTTCCTTCTCTTTCGTTTTATAGGCTGACCAGGTCTCTTCAACCTCGAT
GTCCCGGTAAGTTCTTTTTAAAC
   
Sequence Name 92-K102438-0022-419-G07C-NS40
GenBank Accession LN515187 [GenBank]
Sequencing primer gene specific primer, NS40: 5'-GGTTTAAAAAGAACTTACCGGGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K102438-0022-419-G07C-NS40
ACGAAAGGAAGGAATAGTTTCGAATCATTAGTAGGGGTATGTTGATAAGAGGAGAAGACA
AAGTGAATGTATTTCTACTTACATGGTTAAGATAGTGCCGTTGGCTATACCGTTGTAATC
ACAAGTTGTAACATTACAGCCAGCGCTAGTCTGCTGCACCGTGATGTTACCGAGCATGAG
GTTGCTGTTTCTACACTGCATACTTGAGCAAGAGACGGCTAGGGAAGCAGGCTCACAATA
CAAACTGCATCACATCACCCACACACAACACAAGTGAGTCAAAATGCAATCAGAGATGAA
TTTGATTAGTCATATCAAATAATAAAGCTTAGATATAATTACTTGCGGCTCCCAAATAAT
AGCTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACA
AATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAAT
CTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11