Sequences from confirmation amplicon


Confirmation sequences for line 420E07 and hit At3g10250 are shown.
Line ID 420E07
Locus At3g10250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3169509
Primers used for PCR 03ek - 8474, show primer details
   
Sequence Name 25-K110713-0022-420-E07C-03ek
GenBank Accession LN497495 [GenBank]
Sequencing primer gene specific primer, 03ek: 5'-AGCATATTTGGAGAAGCATCAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K110713-0022-420-E07C-03ek
TTGTATTGAGTGTGGAAGAGCCATTGAGGAATGCATTAGACAGACTAGAAGCCATTGGAT
GATGTGCACTTTCAGACTTCAAAGATTGGTCAGTGTGCTCAGAGGGATAGCATAATTGTT
TCTGATTCACTGTTAAGAAAATCACATAGATTAGCTATTTAATACTCATCAGTTAGCGAC
TTGAATGAATGACTAATCTAAGGAAAACAACAAACACATAATGTTCACAGGAAAAAGAAA
CCTTGAAAAGAACTAAGGCAGCAAAAGTGGAAGCCAGAAAACAAGACAATGCATATGAGG
AACAATTATGCTATCCCTAAAATGTAAATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 09-K110711-0022-420-E07C-8409
GenBank Accession LN490233 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K110711-0022-420-E07C-8409
GAGCCATTTACAATTGAATTACATTTTAGGGATAGCATAATTGTTCCTCATATGCATTGT
CTTGTTTTCTGGCTTCCACTTTTGCTGCCTTAGTTCTTTTCAAGGTTTCTTTTTCCTGTG
AACATTATGTGTTTGTTGTTTTCCTTAGATTAGTCATTCATTCAAGTCGCTAACTGATGA
GTATTAAATAGCTAATCTATGTGATTTTCTTAACAGTGAATCAGAAACAATTATGCTATC
CCTCTGAGCACACTGACCAATCTTTGAAGTCTGAAAGTGCACATCATCCAATGGCTTCTA
GTCTGTCTAATGCATTCCTCAATGGCTCTTCCACACTCAATACAAACGTGCCCTCTTCTA
TAAACATATCAACCCATGCTAGGAGAGTTGATGCTTCTCCAAATATGC

Last Updated on 06.12.2018 16:11