Sequences from confirmation amplicon


Confirmation sequences for line 420H08 and hit At1g63300 are shown.
Line ID 420H08
Locus At1g63300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23483274
Primers used for PCR 31lm - 8474, show primer details
   
Sequence Name 29-K114022-0022-420-H08C-31lm
GenBank Accession LN499381 [GenBank]
Sequencing primer gene specific primer, 31lm: 5'-TCGTTCTTAAGCTTCTCTACCTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K114022-0022-420-H08C-31lm
CATTCGAACTATTGGTCGAGTCATCAGTGCTGCTGATACCATGATCAGAACTTCCGGACC
ACTCGGACTCCGATATACGAGAAGGCTCTTCAAACAAACTTTTAGCTGAATGAAGATGCT
TAGTGGGATGTCTCAAAGGCTTGGCAACTTCTTCAGGTGTATTTGGCTCAATGACACTTC
CAGAGCTTGACATTGTACTATCAGATTCAATTGATGCTCTTCGTCTCAGTTCCGCAAACC
GCGCAGCTTTACCAAATGGCCCTTCCTATAAATACAACAAAGATAGTAGAACATCAAGAG
AAAATCCAAAAACTCTCTTGCATCACATTGCACTACGATCAGCCAAATCAACATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 08-K114021-0022-420-H08C-8409
GenBank Accession LN489814 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K114021-0022-420-H08C-8409
AATGTGATGCAAGAGAGTTTTTGGATTTTCTCTTGATGTTCTACTATCTTTGTTGTATTT
ATAGGAAGGGCCATTTGGTAAAGCTGCGCGGTTTGCGGAACTGAGACGAAGAGCATCAAT
TGAATCTGATAGTACAATGTCAAGCTCTGGAAGTGTCATTGAGCCAAATACACCTGAAGA
AGTTGCCAAGCCTTTGAGACATCCCACTAAGCATCTTCATTCAGCTAAAAGTTTGTTTGA
AGAGCCTTCTCGTATATCGGAGTCCGAGTGGTCCGGAAGTTCTGATCATGGTATCAGCAG
CACTGATGACTCGACCAATAGTTCGAATGATATAGTCGCGAGGGACACCGCGATTAACTC
CTCGGATGAGGACGAGGTAGAGAAGCTTAAAGAACG

Last Updated on 06.12.2018 16:11