Sequences from confirmation amplicon


Confirmation sequences for line 421C06 and hit At3g16850 are shown.
Line ID 421C06
Locus At3g16850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5749455
Primers used for PCR 30we - 8474, show primer details
   
Sequence Name 28-K118356-0022-421-C06C-30we
GenBank Accession LN499044 [GenBank]
Sequencing primer gene specific primer, 30we: 5'-TTGTCTCCATTGATCAAACTTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K118356-0022-421-C06C-30we
GCGACATAGGACGGTAGAGGACTGACAACGTCCCAATGCGACGGATCCTAGCACATTAGA
CAACAATTGAGAGACCGTGTTACAAAAAACTTGTCTACCAAACACTTCTTAAAGAGCTAT
AGATACCTAAAGAACACTATTTAGCTTTACAAGATTTGCTAGAGAGGTCACTCATACCGG
TGATGCAAGAATTGTTGCTCCTTTCTCCAAGAAGAGAGTGAGATGGCTTGTGAGATTGAA
GCTTCCGGTGAGCCATTTCCCTGGTGGAACATAAAGCTGAGCACCGCCTTTATCCGCAAA
AGACTTGAGATAGAAAACAGCGTTCTGGAAAGCAATAGTGTTGAGCGTCTTCCCATCACC
AACCGCTCCAAACTCGGATACCAATGAATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 28-K118355-0022-421-C06C-8409
GenBank Accession LN499043 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K118355-0022-421-C06C-8409
AGCGGTTGGTGATGGGAAGACGCTCAACACTATTGCTTTCCAGAACGCTGTTTTCTATCT
CAAGTCTTTTGCGGATAAAGGCGGTGCTCAGCTTTATGTTCCACCAGGGAAATGGCTCAC
CGGAAGCTTCAATCTCACAAGCCATCTCACTCTCTTCTTGGAGAAAGGAGCAACAATTCT
TGCATCACCGGTATGAGTGACCTCTCTAGCAAATCTTGTAAAGCTAAATAGTGTTCTTTA
GGTATCTATAGCTCTTTAAGAAGTGTTTGGTAGACAAGTTTTTTGTAACACGGTCTCTCA
ATTGTTGTCTAATGTGCTAGGATCCGTCGCATTGGGACGTTGTCAGTCCTCTACCGTCCT
ATGGTCGCGGAATTGAACTTCCTGGAAAGCGGTACAGAAGTTTGATC

Last Updated on 06.12.2018 16:11