Sequences from confirmation amplicon


Confirmation sequences for line 422C11 and hit At3g43110 are shown.
Line ID 422C11
Locus At3g43110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:15091833
Primers used for PCR JG63 - 8474, show primer details
   
Sequence Name 18-K034065-022-422-C11C-8409
GenBank Accession LN494209 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K034065-022-422-C11C-8409
AAGCCATTTACAATTGAAGGTTGGTACCGTTACGTGGATTTAACGGTGATTAACGGAAAT
GTTGTGAGGTTGTGGGATGCTAACCGTGTACGTAACGGTGGTTGGGTTAGTGTGCAACGT
AAGGAGTGTTATGGTTGAGACATGTTGGGGACAAGTATAATTTTCTAATTTAAGCATATG
ATTATTCATTGAACCTTTTTGGTTTAAAGACTATTTTTGTTGAATTAAAGTTGTAATGTG
TATATATATTTTAGTGATATAACTATGATCATTGTTGAATAAAGAAGAACAAATTCCCTT
TCATGTTTTTTTTTTTTGTTTTTGATTATATTTTTCAACTAAATCACTATCCATGTTACC
CACGGTATTTTTGGTTTAATCAATAATCAAATCTCATTTCTGTGTGTTCCACAAAAACTC
TTTTTTATTTTTATTATCTTTAAACATGTATTTGTTTATCATGTATTATTAGTACCAACT
AAATATATTTAGTCTGGTCAAACTATATTTTGTTTACTATATGTATTTTCCTCGGTTTGT
ACCAGGAAAAATATATTTCTATAGTTGGTATGTA
   
Sequence Name 85-K034065-022-422-C11C-JG63
GenBank Accession LN514497 [GenBank]
Sequencing primer gene specific primer, JG63: 5'-TACGTACCCAAACGTACATACCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K034065-022-422-C11C-JG63
CGAGGAACTACATATAGTAAACAAAATATAGTTTGACCAGACTAAATATATTTAGTTGGT
ACTAATAATACATGATAAACAAATACATGTTTAAAGATAATAAAAATAAAATAGAGTTTT
TGTGGAACACACAGAACTGAGATTTGATTATTGATTAAACCAAAAATACCGTGCGTAACA
TGGATAGTGATCTAGTTGAAAACTATAATCAAAAACAAAAAAAAAAAACATGAAAGGGAA
TTTGTTCTTCTTTATTCAACAATGATCATATTTATATCACTAAAATATATATACACTTTA
CAACTTTAATTCAACAAAAATAGTCTTTAAACCAAAAAGGTTCAATGAAAAATCATATGC
TTAAATTAAAAAATTATACTTGTCCCCAACATGTCTCAACCATAACACTCCTTACTTTGC
ACACTAACCCAACCCCCGTTACGTACACGGTTAGCATCCCACAACCTCACAACATTTCCT
TTAATCACCGTTAAATCCACGTAACGGTACCAACCTTCAATTGTAAATGGCTTCATGTCC
GGAAAATCTACATGGATCACCAATGAGTATAATGGTCAATATGGAAAAAAAAAAAAAGTA
ATTACCAATTTTTTTTCATTTCAAAAATGTAAATTTCCCCACCGTTTTTATAAAATGAAA
GTACATTTTGATAAAACAACAAATTACAATCCGTCGTATTTATAGGCAAAAGCAATAAAC
AAATTATTCTAATTCGAAAATCTTTATTTCAACTTGTCTACTTTCCTCCCC

Last Updated on 06.12.2018 16:11