Sequences from confirmation amplicon


Confirmation sequences for line 422D08 and hit At5g49270 are shown.
Line ID 422D08
Locus At5g49270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19973037
Primers used for PCR JC14 - 8474, show primer details
   
Sequence Name 15-K101743-0022-422-D08C-8409
GenBank Accession LN492913 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K101743-0022-422-D08C-8409
GAACGGTACCATCTTACCCGTATCATGGACCCTTCCAATCAGTCTCCGTCTTCACGATGC
AAGTTGCCAAAATGCCTCCTGATTTCAACCGATCCGCGCTTTTCCCACCGCAGAACTGGA
GGATTAAAGGGACGCTAAACCCGGATTACTCGTGCGGGCCACCCGTTCGGGTAACCCCGA
CTTTTTATCCGGATCCGAGTGGTATGCCGACCAACAAATCATCTTTCGCCAGTTGGCAGA
TCGTATGCAACATCACGCAAGCCAAAACCGAGATACCAAAATGTTGTGTCTCCTTCTCTG
CTTTCTTCAACGACTCTATCATCCCTTGTAACACTTGCGCTTGTGGTTGCGTTTCCGAAA
CGCGACGCACATGCAGTGCAGAAACTCCGTCTCTTCTCATCCCTCCAGACGCTCTTCTCT
TACCTTTCGAAAACAGAACCGCTCTAACGCTTGCTTGGAACGCACTCAAACACAAAACCC
TACCTAATCCAATGCCGTGTGGGGACAATTGCGGTGTATCGATTAACTGGCACATGGCGT
CGGATTATAGAGGTGGTTGGACAGTGAGGATCACGATTTTCAACTGGGGAGAGATAGATT
TTCC
   
Sequence Name 15-K101749-0022-422-D08C-JC14
GenBank Accession LN492914 [GenBank]
Sequencing primer gene specific primer, JC14: 5'-AAGGAACCAGTTGGGAAAATCTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K101749-0022-422-D08C-JC14
ACCACCTCTATAATCCGACGCCATGTGCCAGTTAATCGATACACCGCAATTGTCCCCACA
CGGCATTGGATTAGGTAGGGTTTTGTGTTTGAGTGCGTTCCAAGCAAGCGTTAGAGCGGT
TCTGTTTTCGAAAGGTAAGAGAAGAGCGTCTGGAGGGATGAGAAGAGACGGAGTTTCTGC
ACTGCATGTGCGTCGCGTTTCGGAAACGCAACCACAAGCGCAAGTGTTACAAGGGATGAT
AGAGTCGTTGAAGAAAGCAGAGAAGGAGACACAACATTTTGGTATCTCGGTTTTGGCTTG
CGTGATGTTGCATACGATCTGCCAACTGGCGAAAGATGATTTGTTGGTCGGCATACCACT
CGGATCCGGATAAAAAGTCGGGGTTACCCGAACGGGTGGCCCGCACGAGTAATCCGGGTT
TAGCGTCCCTTTAATCCTCCAGTTCTGCGGTGGGAAAAGCGCGGATCGGTTGAAATCAGG
AGGCATTTTGGCAACTTGCATCGTGAAGACGGAGACTGATTTGGAAGGGTCCATGATACG
TGGTAAGATGGTACCGTTTCTATGATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
ANAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11