Sequences from confirmation amplicon


Confirmation sequences for line 422D09 and hit At5g11880 are shown.
Line ID 422D09
Locus At5g11880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3829919
Primers used for PCR KF13 - 8474, show primer details
   
Sequence Name 11-K103223-0022-422-D09C-8409
GenBank Accession LN491070 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K103223-0022-422-D09C-8409
GCCATTACAAAATCCTCCAGCCATGGCGGCAGTCGCGGAACCCTAAATCAGTACCAATTG
AATCAGACTTCACTCTCAAGAATCCCATTTTTGTCTCTCAAGTCAACATTGAAGCCACTT
AAACGCCTCTCCGTCAAAGCCGCCGTTTCCCAAAACTCCACCAAAACCCTCACGAAAGAA
TCTGCTTCCTCTTTCGACCACTGTTTCAAGAAATCATCAGATGGGTTTCTCTATTGCGAA
GGAACCAAGGTTCAAGATATCATGGAAACAGTCGAGAAACGACCCTTTTACTTATATAGC
AAACCTCAGATCACGAGAAACCTTGAGGCTTATAAAGAGGCTTTGGAAGGAGTGAGATCT
GTTATTGG
   
Sequence Name 59-K103230-0022-422-D09C-KF13
GenBank Accession LN509971 [GenBank]
Sequencing primer gene specific primer, KF13: 5'-CCAATAACAGATCTCACTCCTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K103230-0022-422-D09C-KF13
TCAGGTTCTCGTGATCTGAGGTTTGCTATATAAGTAAAAGGGTCGTTTCTCGACTGTTTC
CATGATATCTTGAACCTTGGTTCCTTCGCAATAGAGAAACCCATCTGATGATTTCTTGAA
ACAGTGGTCGAAAGAGGAAGCAGATTCTTTCGTGAGGGTTTTGGTGGAGTTTTGGGAAAC
GGCGGCTTTGACGGAGAGGCGTTTAAGTGGCTTCAATGTTGACTTGAGAGACAAAAATGG
GATTCTTGAGAGTGAAGTCTGATTCAATTGGTACTGATTTAGGGTTCCGCGACTGCCGCC
ATGGCTGGAGGATTTTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCTACATTCACGTCCAAATGG
Primers used for PCR KF14 - 8474, show primer details
   
Sequence Name 13-K103223-0022-422-D09C-OriL
GenBank Accession LN491997 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K103223-0022-422-D09C-OriL
TCGGACATGAGCATTTACATTGAATATATCCTGTTAGGATTTTTGAGAAATTGAGTAACT
GCCGCCATGGCTGGAGGATTTTTGTTAGGTGTGCGACTGTTACAGTAACACTGAAGGGTT
TAGAAAAAAAAAGTCAACAAGTTTAAGAGTAGTTCCTGTCGGGTCCGGTTTTAGATATCC
GACAATATCGAACCGGTGGTTATAGTCGAACCGATGGTTAAAAGAAGAAAGAACAAAGTC
GATTTTAACTTCTTTTGGATATGGTTTTTGTCCTTGGGGGATAGTTTCTGCTAGTGTTTG
TTTTGTTCGGTTGTGTTAGTTGTGTGTTGTAATAATCTTGATGCTTAAGGGGTCACCTAA
AGCAGTCATGGAAATCTAGCCCTATCTATAAAAATTTGCCATTTCTCATCACCTACCAAT
ACTTCTATTTGTTCGTATTATGCGGATATTCAGAGGATAATGTAAAACATTTTGTTGAAT
GCTAACGATGAA
   
Sequence Name 61-K103230-0022-422-D09C-KF14
GenBank Accession LN510491 [GenBank]
Sequencing primer gene specific primer, KF14: 5'-CTTCATCGTTAGCATTCAACAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K103230-0022-422-D09C-KF14
ATATCCGCATAAACGAACAATAGAAGTATTGGTAGGTGATGAGAAATGGCAAATTTTTAT
AGATAGGGCTAGATTTCCATGACTGCTTTAGGTGACCCCTTAAGCATCAAGATTATTACA
ACACACAACTAACACAACCGAACAAAACAAACACTAGCAGAAACTATCCCCCAAGGACAA
AAACCATATCCAAAAGAAGTTAAAATCGACTTTGTTCTTTCTTCTTTTAACCATCGGTTC
GACTATAACCACCGGTTCGATATTGTCGGATATCTAAAACCGGACCCGACAGGAACTACT
CTTAAACTTGTTGACTTTTTTTTTCTAAACCCTTCAGTGTTACTGTAACAGTCGCACACC
TAACAAAAATCCTCCAGCCATGGCGGCAGTTACTCAATTTCTCAAAAATCCTAACAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCC
GTCGTATTTATGGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGA
CGTGTCTACTTTCAC

Last Updated on 06.12.2018 16:11