Sequences from confirmation amplicon


Confirmation sequences for line 422D11 and hit At1g21990 are shown.
Line ID 422D11
Locus At1g21990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7741089
Primers used for PCR BC10 - 8474, show primer details
   
Sequence Name 02-K103979-0022-422-D11C-BC10
GenBank Accession LN487005 [GenBank]
Sequencing primer gene specific primer, BC10: 5'-AGCTTCATAAGAGCATTGGTGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K103979-0022-422-D11C-BC10
CGGTGATCCACTTCACGCGCACCTTGACAGTCTCCAGACATTTCAAATTTGCCAAGAAAT
GACTCATCTGCTTCTGCTCACGACAAGTTCCGCGATACCCTATAATCTTTAACACCTTCA
CTTGACATGTCAATAAACAACATTCTCCCCTCGTCTTCTTACGGATGCAAAGGCAGGCGT
CCCCGCATCTACTAGTAACTTTGTGCACAAGTCCCTGAAATCGAAAGGAAGAAATTCATG
TAAAGCAAAAAGAATAACAAGTGTGTGTTTTGATCAACAGAAATTTGATATATACATGTA
TTTACCTTGATGACTAAAGTTTCCAGGTTTGGAGAGCTGTTAAGAAGAAGTGGCACCACT
TGCCAGCCTCTTTCTTTGTCACTCTCAAACGATAACTTAAGGAGGTTTTGAAACGCCGGC
ATTACTTTACACAGAGAGTGAATAACCTGATTTATATCACAAATCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 18-K103980-0022-422-D11C-8409
GenBank Accession LN494277 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K103980-0022-422-D11C-8409
GCCATTTACAATTGATTTGTGATATAAATCAGGTTATTCACTCTCTGTGTAAAGTAATGC
CGGCGTTTCAAAACCTCCTTAAGTTATCGTTTGAGAGTGACAAAGAAAGAGGCTGGCAAG
TGGTGCCACTTCTTCTTAACAGCTCTCCAAACCTGGAAACTTTAGTCATCAAGGTAAATA
CATGTATATATCAAATTTCTGTTGATCAAAACACACACTTGTTATTCTTTTTGCTTTACA
TGAATTTCTTCCTTTCGATTTCAGGGACTTGTGCACAAAGTTACTAGTAGATGCGGGGAC
GCCTGCCTTTGCATCCGTAAGAAGACGAGGGGAGAATGTTGTTTATTGACATGTCAAGTG
AAGGTGTTAAAGATTATAGGGTATCGCGGAACTTGTCGTGAGCAGAAGCAGATGAGTCAT
TTCTTGGCAAATTTGAAATGTCTGGAGACTGTCAAGGTGCGCGTTGAAGTGGATCACCGA
GAAGACAACGATGCTAGTAATACGTACCTGAGAATCACCAATGCTC

Last Updated on 06.12.2018 16:11