Sequences from confirmation amplicon


Confirmation sequences for line 422G03 and hit At5g22760 are shown.
Line ID 422G03
Locus At5g22760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7575527
Primers used for PCR CS62 - 8474, show primer details
   
Sequence Name 29-K104847-0022-422-G03C-8409
GenBank Accession LN499278 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K104847-0022-422-G03C-8409
CATTTACATTGAATATATCCTGAAAGCAGGTATCCGATTATTCTGTATTGGAGCAAAACG
TGCAAGATGTTAAAGGTCCAGAGACAGAGGCCACTATTTTCAGGAACGCTAGTATTTGTG
TCAAGAAGATTATAGATAACAAGGTTAGATACGGAGTTGTCTTTGGGAATCAAAAGCATC
TTCCATCCCGTGTCATGAAGAATGTCATTGAAGTCGAGAAATCTGAAGATAGAAACGAAA
AGTATTGGTTTCATGAAGCACGCGTTCCCTTATATTTGATCAAAGAATATGAAGAAAGTT
TGCATAGGGTGGTACACATACCTTTTATCAAGAAGCCATCAAGAAAATANTCAAAGC
   
Sequence Name 14-K104846-0022-422-G03C-CS62
GenBank Accession LN492497 [GenBank]
Sequencing primer gene specific primer, CS62: 5'-AGCTTTGATATTTTTCTTGATGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K104846-0022-422-G03C-CS62
AACTTTCTTCATATTCTTTGATCAAATATAAGGGAACGCGTGCTTCATGAAACCAATACT
TTTCGTTTCTATCTTCAGATTTCTCGACTTCAATGACATTCTTCATGACACGGGATGGAA
GATGCTTTTGATTCCCAAAGACAACTCCGTATCTAACCTTGTTATCTATAATCTTCTTGA
CACAAATACTAGCGTTCCTGAAAATAGTGGCCTCTGTCTCTGGACCTTTAACATCTTGCA
CGTTTTGCTCCAATACAGAATAATCGGATACCTGCTTTTCAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11