Sequences from confirmation amplicon


Confirmation sequences for line 422H04 and hit At3g24150 are shown.
Line ID 422H04
Locus At3g24150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8725819
Primers used for PCR 20lx - 8474, show primer details
   
Sequence Name 44-K114129-0022-422-H04C-20lx
GenBank Accession LN505260 [GenBank]
Sequencing primer gene specific primer, 20lx: 5'-TTGTCATTTTGTGAAGCTGAAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K114129-0022-422-H04C-20lx
AGAATTGTTAAACCTCGGAATTGGCTTTGAGTACATGATTTCTTCAGCTTTTAACACAAC
CACAGGCAATTTCTCCTGCCATTCTTTGTTCTTCCTCGTCGCTGCGCTATGTGCTTCCAT
TGCGAGTCTGAAATCAAACAAAGAAAACCCCAAATTAAGATACAAAGAAACCCTAGCGTA
CGGTTTTGAGAAAGATGAGTGAATGAAAAAAACCTGAAAATCTGGCGAATAATCGAACCA
CGAATAGGTTGATGACGATCGCTATGCCAAGCTCGTTTCACACACTCGTAAGGTCTTGGT
CCTGGCCTCGGCATTTTCTCCTTCTTCACACACTTGAGCAGTGACACACTCAGCTACAAA
TCGAAGTAACAAGTCACTCACTCATCAAACTAATCGGTTCAATTTCTATCCGGGAGTATT
TTCCGATCAGCGAGTTCGATGTACGAGTCGCCAAATGGTGTGGAAAGAAAAATCAACCTT
AAAACTCTTTTTAACCGATCCAAGGAAACAGATCTGAAGCTTTGAAGCTCTGAATCGTTT
GGAGATTTCGTCTACATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCA
   
Sequence Name 02-K114128-0022-422-H04C-8409
GenBank Accession LN487123 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K114128-0022-422-H04C-8409
ACGAATCTCCAACGATTCAGAGCTTCAAGCTTCAGATCTGTTTCCTTGGATCGGTTAAAA
AGAGTTTTAAGGTTGATTTTTCTTTCCACACCATTTGGCGACTCGTACATCGAACTCGCT
GATCGGAAAATACTCCCGGATAGAAATTGAACCGATTAGTTTGATGAGTGAGTGACTTGT
TACTTCGATTTGTAGCTGAGTGTGTCACTGCTCAAGTGTGTGAAGAAGGAGAAAATGCCG
AGGCCAGGACCAAGACCTTACGAGTGTGTGAAACGAGCTTGGCATAGCGATCGTCATCAA
CCTATTCGTGGTTCGATTATTCGCCAGATTTTCAGGTTTTTTTCATTCACTCATCTTTCT
CAAAACCGTACGCTAGGGTTTCTTTGTATCTTAATTTGGGGTTTTCTTTGTTTGATTTCA
GACTCGCAATGGAAGCACATAGCGCAGCGACGAGGAAGAACAAAGAATGGCAGGAGAAAT
TGCCTGTGGTTGTGTTAAAAGCTGAAGAAATCATGTACTCTAAAGCCAATTCCGAGGTTT
TAAACAAATTTTCCTTCTCTTTTTTTAGTGTGTTTTCAGCTTCACAAAATGAC

Last Updated on 06.12.2018 16:11