Sequences from confirmation amplicon


Confirmation sequences for line 423F11 and hit At4g25370 are shown.
Line ID 423F11
Locus At4g25370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12973698
Primers used for PCR 10ag - 8474, show primer details
   
Sequence Name 15-K106400-0022-423-F11C-8409
GenBank Accession LN492984 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K106400-0022-423-F11C-8409
GATCTGTATAGATAATGCTGGTGAAGATTTCTCTGTCTTGCTTTGTGCTTTAGTCCACCC
TCTTAACTGATTCCGAGCATACCTTATAAGTACAGGCTGTGTTTGTTCCTATAAAACAAA
ATACATGTGAAATAAGGTAACCATAAAAAACTGTAACTTTATTATATTCAGTTGTTGAAT
GCTTATGCGGTTTGATCGAGAGGAGAGAACTGGGTGGAATCATCAGAAAGAAAGTTTTAT
CTTTGTGTTTTATACCCTTCTTCTTTCTGTTATTCAAAAGGCATTTATGGTAAATCGTTT
TGGATTATGTGGTTTACTGACGTATATTAAGCTTGTCATAGTTGGTTTTGATTTTAGGCA
CTAGCACAGTTGCTAAGTTCCTGAGGGGGAATGGAGTCACACTTTTCAAGGTTCGAGATG
AGACGTTAAGCCTGCTGGGGAAATCAGACATGTACTTTTTCAGCCCAGAGCATCCTCCTC
TAACTGAGCCGGCTCAAAAGGCCATTGCCTGGGCCATTGATGAGAAGAATAAATCTGGTG
TGCATAAACAACACCTCCTTAATATTTTGTGGATCCTTGCTGTTTCCCATCCATTATATT
ATATTGCCAACTGAAACTATTGTGGTCACA
   
Sequence Name 31-K106401-0022-423-F11C-10ag
GenBank Accession LN500175 [GenBank]
Sequencing primer gene specific primer, 10ag: 5'-CTGTGACCAAATAGTTTCAGTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K106401-0022-423-F11C-10ag
GGGAACGCAGGATCCACAAAATATTAAGGAGGTGTTGTTTATGCACACCAGATTTATTCT
TCTCATCAATGGCCCAGGCAATGGCCTTTTGAGCCGGCTCAGTTAGAGGAGGATGCTCTG
GGCTGAAAAAGTACATGTCTGATTTCCCCAGCAGGCTTAACGTCTCATCTCGAACCTTGA
AAAGTGTGACTCCATTCCCCCTCAGGAACTTAGCAACTGTGCTAGTGCCTAAAATCAAAA
CCAACTATGACAAGCTTAATATACGTCAGTAAACCACATAATCCAAAACGATTTACCATA
AATGCCTTTTGAATAACAGAAAGAAGAAGGGTATAAAACACAAAGATAAAACTTTCTTTC
TGATGATTCCACCCAGTTCTCTCCTCTCGATCAAACCGCATAAGCATTCAACAACTGAAT
ATAATAAAGTTACAGTTTTTTATGGTTACCTTATTTCACATGTATTTTGTTTTATAGGAA
CAAACACAGCCTGTACTTATAAGGTATGCTCGGAATCAGTTAAGAGGGTGGACTAAAGCA
CAAAGCAAGACAGAGAAATCTTCACCAGCATTATCTTATACAGATCTCTCAACTCAATGT
AAGACTTTTCAGTTTCCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAG

Last Updated on 06.12.2018 16:11