Sequences from confirmation amplicon


Confirmation sequences for line 423G08 and hit At3g58070 are shown.
Line ID 423G08
Locus At3g58070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21507536
Primers used for PCR YG03 - 8474, show primer details
   
Sequence Name 21-K027064-022-423-G08C-8409
GenBank Accession LN495517 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K027064-022-423-G08C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATAAATACTCATTCGCCGTCCTAA
AGATAAGAACCCTAAACCCATTCGTGTCTTCGGAAAAGATTTCACCGGCAGAGATTTCTC
TATTACTACCGGTCAAGAAGACTACACCGATCCTTACCAGACCAAAAACAAAGAAGAAGA
AGAGGAAGAAGACCAAACCGGAGACAACAGTACGGACAATAATAGCATCAGCCACAACAG
GAGATTCGAGTGTCACTATTGCTTTAGAAATTTTCCTACTTCACAAGCCCTAGGTGGACA
CCAAAACGCTCACAAACGCGAACGTCAGCTTGCCAAACGCGGTGTTTCCTCTTACTTTTA
TCATCCTGACAATAACCCTTACAGTTACCGCATCCCG
   
Sequence Name 22-K027064-022-423-G08C-YG03
GenBank Accession LN495963 [GenBank]
Sequencing primer gene specific primer, YG03: 5'-ACGGGTAATGACGGTAACTGTAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K027064-022-423-G08C-YG03
GGTTATTGTCAGGATGATAAAAGTAAGAGGAAACACCGCGTTTGGCAAGCTGACGTTCGC
GTTTGTGAGCGTTTTGGTGTCCACCTAGGGCTTGTGAAGTAGGAAAATTTCTAAAGCAAT
AGTGACACTCGAATCTCCTGTTGTGGCTGATGCTATTATTGTCCGTACTGTTGTCTCCGG
TTTGGTCTTCTTCCTCTTCTTCTTCTTTGTTTTTGGTCTGGTAAGGATCGGTGTAGTCTT
CTTGACCGGTAGTAATAGAGAAATCTCTGCCGGTGAAATCTTTTCCGAAGACACGAATGG
GTTTAGGGTTCTTATCTTTAGGACGGCGAATGAGTATTTATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGCAGCTACATTC

Last Updated on 06.12.2018 16:11