Sequences from confirmation amplicon


Confirmation sequences for line 423H04 and hit At4g19750 are shown.
Line ID 423H04
Locus At4g19750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10746670
Primers used for PCR 57qv - 8474, show primer details
   
Sequence Name 41-K115837-0022-423-H04C-57qv
GenBank Accession LN504204 [GenBank]
Sequencing primer gene specific primer, 57qv: 5'-AGCCAATACTTGGTACTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K115837-0022-423-H04C-57qv
TAGACTAGAACCCAGTCTTTATTTTCTTTTATTAACCGTAGACTTTATTTTTTGTTAATT
TCTAATTTCATGTTGGACAAGCCATATTAAAAAAATAAAAATCCCAACACGAAATTAGAA
ATTGAAATACTATATACAAGNCTTTTTTTTTTGGGCCACTCACATCCTTAGCAAAAACTT
TTGGCTTTCGAAATACTAAACTCAAAGTTGAAGTTGGTTTTTATTTTCCAAAACTTAACC
ATATCATTTCTCTAAACCCTTATAAATAGACAACGATCCCATCCTCATAATTCCAAAACC
CAACCAAACCCGGAAGGCACTTGGCTAAAGACCCTCGACCAAAAGGGCGGATCAAACTCA
GTTTCTCCAAACCAACTCGATGGTAAAAGCTTCCTACGGGGTGGGGAAGCCGGAAAACAA
CTTCCCGGCCGGAAACATGGACTCAACTCGCTTCACCCATCTTTTCGGCGCCTTCGCTGA
CGTCAACCCTTCCACCCAGGAAGTCACTATCTCTGCCGCGAACTCAGGCCAAGTCTCCGG
CTTTACCCATACGGTTAAAGACAAAAACACGGACGTGCAAACTCTCTGGTCAATGGGGGG
TAAAAATGCAAACAAAGCTGTGTTAGCGTCCAGGGCAGGTAACAGCAAAAACCGGAAAGC
GTTCATGGATTCCCCGATCGATATCGCTGGGAAAAAGGATTTCTACGGCCTCAACCTAGC
TGGGGAGTATCCAAGCAAGGACTGGAAGATGGCCAACTTTGGGAAACTCTTGAAAAAAGG
GCAAGCTGCGGTTGTGGAAAAAAAGGAAAAAGAATTGAGTATAAAGGGGCTTATTAGAAA
TATATTCAATTGTAAATGGCTCCAGGTCCGGGAAATCTACTTGGATCAGCATTGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCATTTTTTTTTCATTTCAAAAAG
   
Sequence Name 09-K115833-0022-423-H04C-8409
GenBank Accession LN490333 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K115833-0022-423-H04C-8409
TCATTGAATAATTTCTATAAGCCCTTTTATACTCATTTCTTTTTCTTTTTCTTCCACAAC
CGCAGCTCGCCATTCTTTCACGAGTTTCCCAAAGTTGGCCATCTCCACGTCATTGCTTGG
ATACTCCCAAGCTAGGTCGAGGCCGTAGAAATCCTTCTTCCTAGCGATATCGATCGAGGA
ATCAATGAACGCTTTCCGGTTTTTGCTGTTACTTGCCATGGACGCTAACACAGCTTTGTC
TGCATCTTTACCCCCGATCGACAAGAGAGTTTGCACGTCCGTGTTTTTGTCTTTAACGGT
ATGGGTAAAGCTGGAGACTTGGCATGAGTTCGCGGCAGAGATAGTGACTTCATGGGTGGA
AGAGTCGACGTCAGCGAAGGCGCAGAAAAGATGGGTGAAGCGAGTTGAGTCAATGTTTCC
GGCAGGGAAGTCGTTTTCCGGCTTCACAACCCAGTAGGAAGCTTTTACGATCGAGTTGGT
TCGGAGAAACTGAGTTTGATCAGCCATTTTGGTCGAGGGTCTTTAGCAAAGTGCTTTCTG
TGTTTGTTTGTGTTTTGTAATTATGAGGATGGTATCGTTGTCTATTTATAAGGGTTTAGA
GAAATGATATGGTTAAGTTTTGGAAAATAAAAACAAACTTCAACTTTGAGTTTAGTATTT
CGAAAGCAAAAAGTCTTTGCTAAGGATGTGAGTGGCACAAAAAAAAAAGCTGGTATATGG
TATTTCAATTTCTAATTTCGGGTGGGGATTTTTATTTTTTTAATATGGCTTGTCCAACTT
GAAATTAGAATTTAACAAAAATTAAGGTCTACGGTTATTAAAAAAAAAATAAAAACGGGG
TTTCTTAGTCTATATATCTAACAATTTATCTGTTAACGT

Last Updated on 06.12.2018 16:11