Sequences from confirmation amplicon


Confirmation sequences for line 424B03 and hit At1g50120 are shown.
Line ID 424B03
Locus At1g50120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18565633
Primers used for PCR 16yd - 8474, show primer details
   
Sequence Name 20-K119741-0022-424-B03C-16yd
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 16yd: 5'-TTCAGATGCCATTAGGTGACAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K119741-0022-424-B03C-16yd
CATCACCGNACGCGAGAGAGATAGGGAGAGAGAAGCTTAGTGCAAGGAGAGAAAAATGGA
GCGCAGAGACGATAGGGTTTTTTTCTTTTTCTTTTCCCTCTCAATGTTGTATTAATATAG
AACAAGTAAAGACATCTAGGGTTTTTTCCAATTTTCCAACTTTTCTATTAATAATTTTTT
TTTTTTTTTTTTTGGGGGGGATAAAAATTTTGAAATTGGGTTTTTCCACCATTTTCTTTA
CCCCTTTTTTCAAAATTTTTTTTTACAAAAATTTCTTTTTGGGCCCCAAAACGGAAAAAC
TGGTTTTAATTACCCTTTTTTAAAAAAACGTTTGGGGATAAAAAATTTAAAATTTTTTTC
CAAGGGACCCCAAAAAAAAAAAAAATTCGCCCCCCTATGGCCAAAAAACCTAGAATTTAA
AACCTAACCAAAAGGGGTTGGGGGAAAATATTTTCCCCTGAAAAATTTTTTTTTAAGGGT
CCAAAAAAAAAACAATTTTTTGCTGGGGGGGGCCTAAACAAAACCCAAAATTTGTTTTTT
TCCCCAAAAAAAATTTTTTTTACAAACCCAAAAGGGCCCCAAACAAAAAAGTTTTTCCCC
CGGTTTTTAACCCCTTAATTTCCAAGGGGGGGGGCCTTTTCCGTAAATCGAAAAAACCCT
TTCCTTTTTTCCCTTTTCGGGAACAAAAAAGGGAGGTCCCACCCGGAAATTTCCCCCAAA
CCAAACCCAAAA
   
Sequence Name 08-K119740-0022-424-B03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K119740-0022-424-B03C-8409
ACCTTATTCAACCAGCTTTCTATTTTCATTCCGACGGATGGTCTCATGTCGTTTTCTACG
CCCCGTGTTTCTGTGCAATGGATTCTTCGGTTTGAGTTCCTAACTACTCCTAAGAGTGTG
GACTTAAGCAGGTATTACTCCCAATACTCAAACGATCCATTCTCTGGTTGAGTCTTTTGA
AGTGCTCAAGTTGTTGAAGTCAGTTTCTGGTTCTGTTTTGTGGTAATTTACAGGTACGAA
CATCCATTGTTAGTACCGGAAAGGGAAAAAAGCGAATGGGTTCTTCCGATTACTGTACAT
GCACCGCCGCCTCGGACATCAGGTGCTCAAAACCGTGGAGATAAGCTTTTTGGTTTGGAG
CCGTCTTGGATTCGTAGCTAAATTTGTTTTTGGTGAAAGATACAGTTTCTGGTCTCTGTC
TAGCTCACCTCATGCAATAATTTGTTTTTCTTTTGATCATTTAAAAATAACTTTTCAAGG
GAAATATATATACCACAAACCTATTTTGTTTAGTTTTTAATTACTAGTTTATTAGACTAT
AGAGAGCAGATTTATTTTTCTTTTTGGTGTCCTATGATAAAAGATCTTAAATCTTTAATC
ACAAACTGTTTCTTATATAAGCGTTACTTAAATCAGTTTCATCAGTTTTGGGCACATAAA
GTAACTTTTGATAAACAAAATTGTAGAAAAATGGGTAAGAGAAACTGATGATAAATCCAA
ATCTCAAAATCTTAATCCTACCAAAAAAAAAAAAAAAAAAATTTTTTAATAAAAATTTGG
AAAATTTGAAA

Last Updated on 06.12.2018 16:11