Sequences from confirmation amplicon


Confirmation sequences for line 424B08 and hit At3g12750 are shown.
Line ID 424B08
Locus At3g12750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4052410
Primers used for PCR FV22 - 8474, show primer details
   
Sequence Name 93-K033882-022-424-B08C-FV22
GenBank Accession LN515224 [GenBank]
Sequencing primer gene specific primer, FV22: 5'-CCTAGGACGAGAGAGAGATAAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K033882-022-424-B08C-FV22
GCAAATCTTGGATTCATAAAATCAGTAGCAAGAAGATCAACAAGTGACATGTATATGAGT
ATCCCAGCAGATGCAGCATTCAACATTCCTTCCACCATTATTGCCTCTTTGCTCTCTTTC
CTGTAGCCTAAACCACTTGACATCCCCAACCCTATTCCTATCCCAAGTGGCGCCGTCACC
GAGAAAAATGTCGCCATTAGCACTGTCGATTTCGACTTCATATCCGCCTATTCATATACA
CACAACATGATCATCATGCTATCATCAATCAATTGAATTTTGAACAATGAAGAAAAAATG
ATTGTTGTGACACGATTCGTCTAAGCTAAGCTACCTATGACCATAAACGATTTGGAAGAG
ACTAACCAGGGAGATGAGTGCAGCAATGAGCAATGATAGCTTCATGTCTACATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTAT
TTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT
ACATTCA
   
Sequence Name 37-K033882-022-424-B08C-8409
GenBank Accession LN502523 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K033882-022-424-B08C-8409
GAAGCCATTTACAATTGAATATATGTAGACATGAAGCTATCATTGCTCATTGCTGCACTC
ATCTCCCTGGTTAGTCTCTTCCAAATCGTTTATGGTCATAGGTAGCTTAGCTTAGACGAA
TCGTGTCACAACAATCATTTTTTCTTCATTGTTCAAAATTCAATTGATTGATGATAGCAT
GATGATCATGTTGTGTGTATATGAATAGGCGGATATGAAGTCGAAATCGACAGTGCTAAT
GGCGACATTTTTCTCGGTGACGGCGCCACTTGGGATAGGAATAGGGTTGGGGATGTCAAG
TGGTTTAGGCTACAGGAAAGAGAGCAAAGAGGCAATAATGGTGGAAGGAATGTTGAATGC
TGCATCTGCTGGGATACTCATATACATGTCACTTGTTGATCTTCTTGCTACTGATTTTAT
GAATCCAAGATTGCAATCCAATCTCTGGCTTCACTTGGCTGCTTATCTCTCTCCGNTCCT
AG
Primers used for PCR FV23 - 8474, show primer details
   
Sequence Name 94-K033882-022-424-B08C-FV23
GenBank Accession LN515277 [GenBank]
Sequencing primer gene specific primer, FV23: 5'-TAGCTTTACTTCTTGTAGCCGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>94-K033882-022-424-B08C-FV23
TTGATCGGGAAAGGATTCCGGCGTTACAACCGGAAAATGATATCTTCTTCCCGGAGAAAG
CTTTTGCTGCAGGAGTGATCCTCTGCACCCGTTACGTTCATATCTTACCAGACGCGTTCG
AGAGATTGAGCTCTCCATGTCTTGAGGACACTACAGCTGGGAAGTTCCCGTTTGCTGGTT
TTGTAGCGATGCTGTCGGCGATGGGGACTCTTATGATCGACACATTCGCGACAGGGTATT
ACAAGAGGCAACATTTTAGCAATAACCATGGGAGCAAGCAAGTGAACGTAGTAGTAGATG
AAGAAGAGCATGCGGGTCATGTTCACATTCACACGCACGCTAGTCACGGACACACACATG
GTTCGACCGAGTTGATCAGAAGACGTATAGTGTCGCAGGTGCTTGAGATTGGGATAGTTG
TGCATTCGGTTATTATAGGGATATCACTTGGAGCTTCACAGAGCATAGACACCATAAAGC
CACTCATGGCTGCACTATCTTTCCATCAGTTCTTTGAAGGTCTTGGCTTCCAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGAT
AAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAA
TTCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 38-K033882-022-424-B08C-Oril
GenBank Accession LN502895 [GenBank]
Sequencing primer gene specific primer, Oril: unknown
Sequence (A. th genome BLAST matches underlined) 
>38-K033882-022-424-B08C-Oril
CAAGAACTGATGGAAAGATAGTGCAGCCATGAGTGGCTTCCCCGAAAAAATGCTCTGTGA
AGCTCCAAGTGATATCCCTCCAAAAAAAGAATGCACAACTATCCCAATCTCAAGCACCTG
CGACACTATACGTCTTCTGATCAACTCGGTCGAACCATGTGTGTGTCCGTGACTAGCGTG
CGTGTGAATGTGAACATGACCCGCATGCTCTTCTTCATCTACTACTACGTTCACTTGCTT
GCTCCCATGGTTATTGCTAAAATGTTGCCTCTTGTAATACCCTGTCGCGAATGTGTCGAT
CATAAGAGTCCCCATCGCCGACAGCATCGCTACAAAACCAGCAAACGGGAACTTCCCAGC
TGTAGTGTCCTCAAGACATGGAGAGCTCAATCTCTCGAACGCGTCTGGTAAGATATGAAC
GAAACCTGTGCAGAGGATCACTCCTGCAGCAAAAGCTTTCACCATGAAGAAGATATCATT
TTCCGGTTGTAACGCCGGAATCCTTTTCCCGATCAACGGTAGACTCACGCCGACTCCTCC
GGCTACAAGAAGTAAAGCT

Last Updated on 06.12.2018 16:11