Sequences from confirmation amplicon


Confirmation sequences for line 425C03 and hit At2g42330 are shown.
Line ID 425C03
Locus At2g42330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17632391
Primers used for PCR PB36 - 8474, show primer details
   
Sequence Name 01-K037024-022-425-C03C-8409
GenBank Accession LN486484 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K037024-022-425-C03C-8409
GCCATTTACATGATCCATATTTCGCCCATTCATCTAATGACTGATTTGATGGAGAGATTC
TTTTTCCCCAAGTGGCTGGATGTTTTATATCACTGGTTGTGTTCAGAACCAAAGTTCGAT
GAGATAATGAAATGGTTTTTGGGTTGGAAAGGAACATTCCCACAGGAGCTTTCTGCAAAC
AGGAGGATCGAAATCCAGTTCAAGCGTGGTCTAGATATGGCGAGGGAAGCAGTTGAACGG
ATGGAAATGTCTCAGCCAGGGGCAAGGGAAAACATAAGTTACCACAAGGCACAAGAGCAG
AGGCAGTCTGAGGGTCGTGCTAAGGTTCAGGCTCAGGTGGATGACCCGGAGGAGCTGAGT
TTTAAAGAAGC
   
Sequence Name 02-K037024-022-425-C03C-PB36
GenBank Accession LN486940 [GenBank]
Sequencing primer gene specific primer, PB36: 5'-TGCTTCTTTAAAACTCAGCTCCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K037024-022-425-C03C-PB36
ACCCTCAGACTGCCTCTGCTCTTGTGCCTTGTGGTAACTTATGTTTTCCCTTGCCCCTGG
CTGAGACATTTCCATCCGTTCAACTGCTTCCCTCGCCATATCTAGACCACGCTTGAACTG
GATTTCGATCCTCCTGTTTGCAGAAAGCTCCTGTGGGAATGTTCCTTTCCAACCCAAAAA
CCATTTCATTATCTCATCGAACTTTGGTTCTGAACACAACCAGTGATATAAAACATCCAG
CCACTTGGGGAAAAAGAATCTCTCCATCAAATCAGTCATTAGATGAATGGGCGAAATATG
GATCATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCG
TATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTG
TCTACATTCACGTCCAAATGG

Last Updated on 06.12.2018 16:11