Sequences from confirmation amplicon


Confirmation sequences for line 425C11 and hit At2g17830 are shown.
Line ID 425C11
Locus At2g17830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7754587
Primers used for PCR JY05 - 8474, show primer details
   
Sequence Name 37-K025675-022-425-C11C-8409
GenBank Accession LN502478 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K025675-022-425-C11C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCATGAATATATTGTCTTTCA
CTGCGATGGTTTACTCTTATGCATCACCAAAGACCTCAACTTTAGGCTTGTGGTTTGGAA
CCCTTATTTTGGGCAAACAAGATGGATCCAACCCAGAAATTCTTACCACATAAAGGACAT
TTACGCTATTGGGTACGATGAGAATAAGAACCACAAAATCCTGAGATTGAAGGATCAGTA
TTACGATTATGATTCACATCCACGGGCACGGATTTGTGAGTTTGAACTCTACAGTTTTGA
GTCTAATTCATGGAAGGTTGTTCTTGATGTTAGTCCTGACTGGTATATACACTCTTATCA
CCGTGGCTTGTCTGTAAAGGGGAATACTTACTGGTATGCAACAGAGAAGCATGGCTATGT
TAATTTCTTGATCTGTTTTGATTTCACAACTGAGAAATTTGGACCGCGTTTGCCTCTGCC
TTTTAACGCTACCGAGTCTTTTACTTATGATGATGTTGTTACTCTCTCTAGTGTTGGAGA
AGAGCAGCTTGCGCTGTTGTTTCAGAGTGATGCTAC
   
Sequence Name 38-K025675-022-425-C11C-JY05
GenBank Accession LN502853 [GenBank]
Sequencing primer gene specific primer, JY05: 5'-CTCCATCATCAATGTAGCATCACT-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K025675-022-425-C11C-JY05
AACAGCGCAAGCTGCTCTTCTCCAACACTAGAGAGAGTAACAACATCATCATAAGTAAAA
GACTCGGTAGCGTTAAAAGGCAGAGGCAAACGCGGTCCAAATTTCTCAGTTGTGAAATCA
AAACAGATCAAGAAATTAACATAGCCATGCTTCTCTGTTGCATACCAGTAAGTATTCCCC
TTTACAGACAAGCCACGGTGATAAGAGTGTATATACCAGTCAGGACTAACATCAAGAACA
ACCTTCCATGAATTAGACTCAAAACTGTAGAGTTCAAACTCACAAATCCGTGCCCGTGGA
TGTGAATCATAATCGTAATACTGATCCTTCAATCTCAGGATTTTGTGGTTCTTATTCTCA
TCGTACCCAATAGCGTAAATGTCCTTTATGTGGTAAGAATTTCTGGGTTGGATCCATCTT
GTTTGCCCAAAATAAGGGTTCCAAACCACAAGCCTAAAGTTGAGGTCTTTGGTGATGCAT
AAGAGTAAACCATCGCAGTGAAAGACAATATATTCATGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11