Sequences from confirmation amplicon


Confirmation sequences for line 425D02 and hit At3g48780 are shown.
Line ID 425D02
Locus At3g48780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18092156
Primers used for PCR BC22 - 8474, show primer details
   
Sequence Name 35-K103981-0022-425-D02C-BC22
GenBank Accession LN501808 [GenBank]
Sequencing primer gene specific primer, BC22: 5'-ACCTCCTATGTTCAACGGATAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K103981-0022-425-D02C-BC22
CGGTTTCCAGCTCACCCCTATTGCTAGCCAGGGCTCGTATTTGCATATCCGCATCTCACT
CAAGAGAAGATCTTATAAAAGCCCTACAGGTAAATGAAATCTTGCATTGAGTTCACTGGT
TCTCACTCCCATATCATTTACCACGAAGAGCCCTAGTAGTATTAGTATAAAACACGAGTT
CTCGGGTTTCTAGATAGGTCTATGTAAATAAAGCCTTTCTCAGAAAGGGAATCTGCAGTT
CATCCACAAGATGGATTCACACGTTTTAATATTCTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 51-K103982-0022-425-D02C-8409
GenBank Accession LN507484 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K103982-0022-425-D02C-8409
AACGTGTGAATCCATCTTGTGGATGAACTGCAGATTCCCTTTCTGAGAAAGGCTTTATTT
ACATAGACCTATCTAGAAACCCGAGAACTCGTGTTTTATACTAATACTACTAGGGCTCTT
CGTGGTAAATGATATGGGAGTGAGAACCAGTGAACTCAATGCAAGATTTCATTTACCTGT
AGGGCTTTTATAAGATCTTCTCTTGAGTGAGATGCGGATATGCAAATACGAGCCCTGGCT
AGCAATAGGGGTGTAGCTGGGAAACCGACGACCACCACCGCCAACTGTTCACAAATTTCA
TTATCCGTTGAACATAGGAGG

Last Updated on 06.12.2018 16:11