Sequences from confirmation amplicon


Confirmation sequences for line 426D03 and hit At5g47070 are shown.
Line ID 426D03
Locus At5g47070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19119513
Primers used for PCR 14jr - 8474, show primer details
   
Sequence Name 36-K113214-0022-426-D03C-8409
GenBank Accession LN502293 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K113214-0022-426-D03C-8409
GCCATTTACAATTGAATATATCCGGTCGTGATAAGCGCCTGTCTGGCGGATAAGGTGTTT
CCCGCCTTCGGATTTACAATTGTCGTGTTAGCCATTTATAATTGTCACTTTAAACATTAA
GTTATATTAAATGTGGTCATAGAGACATGATTAGTAGTTTAGGATCGAGACTTCTGTGAA
CCGGGGATGTGTGTTTAGTGTTTAGATTGGAAGATAATTTCCTATTTATTTGGTTGACAG
GTGATATATAGAGATTTCAAATCCTCTAATGTGCTTTTAGACGATCAATTCTGTCCAAAA
TTATCGGACTTTGGGCTTGCCAGAGAAGGACCTGATGGCGACAATACTCATGTCACTACC
GCAG
   
Sequence Name 36-K113215-0022-426-D03C-14jr
GenBank Accession LN502294 [GenBank]
Sequencing primer gene specific primer, 14jr: 5'-CTGCGGTAGTGACATGAGTATTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K113215-0022-426-D03C-14jr
CAAGTCCGATATTTTGGACAGAATTGATCGTCTAAAGCACATTAGAGGATTTGAAATCTC
TATATATCACCTGTCAACCAAATAAATAGGAAATTATCTTCCAATCTAAACACTAAACAC
ACATCCCCGGTTCACAGAAGTCTCGATCCTAAACTACTAATCATGTCTCTATGACCACAT
TTAATATAACTTAATGTTTAAAGTGACAATTATAAATGGCTAACACGACAATTGTAAATC
CGAAGGCGGGAAACACCTTATCCGCCAGACAGGCGCTTATCACGACCGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATT

Last Updated on 06.12.2018 16:11