Sequences from confirmation amplicon


Confirmation sequences for line 426H08 and hit At5g24420 are shown.
Line ID 426H08
Locus At5g24420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8336824
Primers used for PCR SD19 - 8474, show primer details
   
Sequence Name 06-K103500-0022-426-H08C-SD19
GenBank Accession LN488776 [GenBank]
Sequencing primer gene specific primer, SD19: 5'-GAGAAGCAGAAATGGGTCACTTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K103500-0022-426-H08C-SD19
AAACACCACCAAAGAGAATCCATTCACTTTACCGGTCATCAACTCTACTTTGTACAATCT
TATGGCCATTTGTGACAAAGCACCGGCCAAATCCGTGGCTGAGATCATGAAGCACAACAA
CCTCTCGTTACCTTCTGCTCATCTTAGTGCTCAAGTCGAAAATGTTTGGTACCTTGACCA
AGCAGCTGCCTCCGAGCTCTAGAGAGGTAGCCTAAACACATTAATATTGGTGTTTTGTTT
GTAGTCTCTACGTTGCTTTGTGTTGAATAACCGGTTAATACATTTGCGTACGTTTACTTC
GTACAAAATGTGTTGAATATTACCAATAGCCAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATATCCGCACGGTTATT
   
Sequence Name 22-K103501-0022-426-H08C-8409
GenBank Accession LN496079 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K103501-0022-426-H08C-8409
GCCATTTACAATTGAATATATCCTGGGCTATTGGTAATATTCAACACATTTTGTACGAAG
TAAACGTACGCAAATGTATTAACCGGTTATTCAACACAAAGCAACGTAGAGACTACAAAC
AAAACACCAATATTAATGTGTTTAGGCTACCTCTCTAGAGCTCGGAGGCAGCTGCTTGGT
CAAGGTACCAAACATTTTCGACTTGAGCACTAAGATGAGCAGAAGGTAACGAGAGGTTGT
TGTGCTTCATGATCTCAGCCACGGATTTGGCCGGTGCTTTGTCACAAATGGCCATAAGAT
TGTACAAAGTAGAGTTGATGACCGGTAAAGTGAATGTGATTCTCTTTGGTGGTGGTTTTG
GAGAGTCGGTGATGTAAGTGACCCATTTCTGCTTC

Last Updated on 06.12.2018 16:11