Sequences from confirmation amplicon


Confirmation sequences for line 428G03 and hit At1g13370 are shown.
Line ID 428G03
Locus At1g13370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4588197
Primers used for PCR JG57 - 8474, show primer details
   
Sequence Name 88-K034065-022-428-G03C-JG57
GenBank Accession LN514822 [GenBank]
Sequencing primer gene specific primer, JG57: 5'-GTTAAAAAGCGACAAAGCAAAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K034065-022-428-G03C-JG57
AAAAAAAAATACTAGGTTCATATGAATGGATTAAATTCTAATTTCTAAGCACGCTCTGCA
CGAATCCTTCTTGCCAATTGAACATCTTTAGGCATAATGGTAACCCTCTTTGCATGAATG
GCACAAAGATTTGTGTCTTCAAACAAACCAACCAAATATGCCTCCGCAGCTTCTTGAAAA
GCTAACACTGCAGGGCTTTGGAATCTCAAATCCGTCTACATTGCAAATTTCCACCAATTA
TTTATCAATCTTTTTCATCAAGTTAGGTATAACTAATCAGTATCTAGGGTTTCGATGATT
GAAAAACAAAATCTTTGAAGCTACATATTCAATTGAATATATCCAGGATATATGTAGGAT
ATAATCAGTATCTTCAATTTACAATCTATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAAAAAAAAAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAAATGTCCGCAGCGTTATTATAAAATGAAAGTACA
TTTTGATAAAACGACAAATTACAATCCGTCGTATTTATAGGCAAAAGCAATAAACAAATT
ATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACTTTCACGTCCAAAATG
   
Sequence Name 21-K034065-022-428-G03C-8409
GenBank Accession LN495574 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K034065-022-428-G03C-8409
AAGCCATTTACAATTGAATATAGATTGTAAATTGAAGATACTGATTATATCCTACATATA
TCCTGGATATATTCAATTGAATATGTAGCTTCAAAGATTTTGTCTTTCAATCATCGAAAC
CCTAGATACTGATTAGTTATACCTAACTTGATGAAAAAGATTGATAAATAATTGGTGGAA
ATTTGCAATGTAGACGGATCTGAGATTCCAAAGCCATGCAGTGTTAGCTCTTCAAGAAGC
TGCGGAGGCATATTTGGTTGGTTTGTTTGAAGACACAAATCTCTGTGCCATTCATGCAAA
GAGGGTTACCATTATGCCTAAAGATGTTCAATTGGCAAGAAGGATTCGTGCAGAGCGTGC
TTAGAAATTAGAATTTAATCCATTCATATGAACTAGTATTTTTTTTTTTCTATTAGGGTT
TTCGATTGTTTTTGCTTTGTCGCTTTTTTAAAAA

Last Updated on 06.12.2018 16:11